- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA447Hu
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- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
C-肽(CP)检测试剂盒
检测范围 123.46-10000pg/mL 灵敏度 45.5pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
C-肽(CP)检测试剂盒
yb-E11901 小鼠巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒 Murine macrophage colony-stimulating factor (M-CSF) ELISA kit
yb-E11902 小鼠巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒 Mouse macrophage -derived chemokine (MDC/CCL22) ELISA kit
yb-E11903 小鼠正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒 Normal T cell expressed and secreted factor (RANTES/CCL5) ELISA kit for mouse
yb-E11904 小鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒 Murine macrophage inflammatory protein- 2 (MIP-2) ELISA Kit
yb-E11905 小鼠催产素(OT)ELISA试剂盒 Mice oxytocin (OT) ELISA Kit
yb-E11906 小鼠Ⅰ型胶原N末端肽(NTX)ELISA试剂盒 Mouse N -terminal peptide of collagen type Ⅰ (NTX) ELISA Kit
yb-E11907 小鼠5核苷酸酶(5-NT)ELISA试剂盒 Mice 5 nucleotidase (5-NT) ELISA kit
yb-E11908 小鼠神经生长因子(NGF)ELISA试剂盒 Mouse nerve growth factor (NGF) ELISA kit
yb-E11909 小鼠神经营养因子3(NT-3)ELISA试剂盒 Mice neurotrophic factor 3 (NT-3) ELISA kit
yb-E11910 小鼠神经营养因子4(NT-4)ELISA试剂盒 Mouse neurotrophin 4 (NT-4) ELISA Kit
yb-E11911 小鼠骨保护素(OPG)ELISA试剂盒 Mice osteoprotegerin (OPG) ELISA kit
yb-E11912 小鼠B因子(BF)ELISA试剂盒 Mouse factor B (BF) ELISA Kit
yb-E11913 小鼠多巴胺D2受体(D2R)ELISA试剂盒 Dopamine D2 receptor mice (D2R) ELISA Kit
yb-E11914 小鼠多巴胺D3受体(D3R)ELISA试剂盒 Mice dopamine D3 receptor (D3R) ELISA kit
yb-E11915 小鼠多巴胺D4受体(D4R)ELISA试剂盒 Mice dopamine D4 receptor (D4R) ELISA kit
yb-E11916 小鼠多巴胺D5受体(D5R)ELISA试剂盒 Mice dopamine D5 receptor (D5R) ELISA Kit
yb-E11917 小鼠甲肝lgG（HAV-IgG）ELISA试剂盒 Mouse hepatitis lgG (HAV-IgG) ELISA Kit
yb-E11918 小鼠戊肝lgG（HEV-IgG）ELISA试剂盒 HEV mouse lgG (HEV-IgG) ELISA Kit
yb-E11919 小鼠戊肝lgA（HEV-IgA）ELISA试剂盒 Mouse hepatitis E lgA (HEV-IgA) ELISA kit
yb-E11920 小鼠癌胚抗原（CEA）ELISA试剂盒 Mouse carcinoembryonic antigen (CEA) ELISA kit
yb-E11921 小鼠白细胞介素-6（IL-6）ELISA试剂盒 Mouse interleukin -6 (IL-6) ELISA Kit
yb-E11922 小鼠晚期糖基化终末产物 (AGEs)ELISA试剂盒 Sugar Mice advanced glycation end products (AGEs) ELISA kit
yb-E11923 小鼠胰岛素(Insulin)ELISA试剂盒 Mouse insulin (Insulin) ELISA Kit
yb-E11924 小鼠低密度脂蛋白（LDL)ELISA试剂盒 Mice low-density lipoprotein (LDL) ELISA kit
yb-E11925 小鼠高密度脂蛋白(HDL)ELISA试剂盒 Mice high-density lipoprotein (HDL) ELISA kit
C-肽(CP)检测试剂盒
检测范围 123.46-10000pg/mL 灵敏度 45.5pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
C-肽(CP)检测试剂盒
ELISA Kit for C-Peptide (CP)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | CEA447Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 2.5 hours |
| Detection range | 123.46-10000pg/mL The standard curve concentrations used for the ELISA’s were 10000pg/mL, 3333.33pg/mL, 1111.11pg/mL, 370.37pg/mL, 123.46pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 45.5pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of C-Peptide (CP).
No significant cross-reactivity or interference between C-Peptide (CP) and analogues was observed.
No significant cross-reactivity or interference between C-Peptide (CP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant C-Peptide (CP) and the recovery rates were calculated by comparing the measured value to the expected amount of C-Peptide (CP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-103 | 93 |
| EDTA plasma(n=5) | 78-89 | 82 |
| heparin plasma(n=5) | 80-93 | 84 |
Precision
C-肽(CP)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Peptide (CP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Peptide (CP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Peptide (CP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-Peptide (CP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-93% | 84-97% | 95-103% | 90-101% |
| EDTA plasma(n=5) | 96-103% | 80-90% | 78-90% | 78-90% |
| heparin plasma(n=5) | 93-101% | 99-105% | 80-99% | 83-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
C-肽(CP)检测试剂盒This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to C-Peptide (CP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled C-Peptide (CP) and unlabeled C-Peptide (CP) (Standards or samples) with the pre-coated antibody specific to C-Peptide (CP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of C-Peptide (CP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of C-Peptide (CP) in the sample.
yb-E11901 小鼠巨噬细胞集落刺激因子(M-CSF)ELISA试剂盒 Murine macrophage colony-stimulating factor (M-CSF) ELISA kit
yb-E11902 小鼠巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒 Mouse macrophage -derived chemokine (MDC/CCL22) ELISA kit
yb-E11903 小鼠正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒 Normal T cell expressed and secreted factor (RANTES/CCL5) ELISA kit for mouse
yb-E11904 小鼠巨噬细胞炎性蛋白2(MIP-2)ELISA试剂盒 Murine macrophage inflammatory protein- 2 (MIP-2) ELISA Kit
yb-E11905 小鼠催产素(OT)ELISA试剂盒 Mice oxytocin (OT) ELISA Kit
yb-E11906 小鼠Ⅰ型胶原N末端肽(NTX)ELISA试剂盒 Mouse N -terminal peptide of collagen type Ⅰ (NTX) ELISA Kit
yb-E11907 小鼠5核苷酸酶(5-NT)ELISA试剂盒 Mice 5 nucleotidase (5-NT) ELISA kit
yb-E11908 小鼠神经生长因子(NGF)ELISA试剂盒 Mouse nerve growth factor (NGF) ELISA kit
yb-E11909 小鼠神经营养因子3(NT-3)ELISA试剂盒 Mice neurotrophic factor 3 (NT-3) ELISA kit
yb-E11910 小鼠神经营养因子4(NT-4)ELISA试剂盒 Mouse neurotrophin 4 (NT-4) ELISA Kit
yb-E11911 小鼠骨保护素(OPG)ELISA试剂盒 Mice osteoprotegerin (OPG) ELISA kit
yb-E11912 小鼠B因子(BF)ELISA试剂盒 Mouse factor B (BF) ELISA Kit
yb-E11913 小鼠多巴胺D2受体(D2R)ELISA试剂盒 Dopamine D2 receptor mice (D2R) ELISA Kit
yb-E11914 小鼠多巴胺D3受体(D3R)ELISA试剂盒 Mice dopamine D3 receptor (D3R) ELISA kit
yb-E11915 小鼠多巴胺D4受体(D4R)ELISA试剂盒 Mice dopamine D4 receptor (D4R) ELISA kit
yb-E11916 小鼠多巴胺D5受体(D5R)ELISA试剂盒 Mice dopamine D5 receptor (D5R) ELISA Kit
yb-E11917 小鼠甲肝lgG（HAV-IgG）ELISA试剂盒 Mouse hepatitis lgG (HAV-IgG) ELISA Kit
yb-E11918 小鼠戊肝lgG（HEV-IgG）ELISA试剂盒 HEV mouse lgG (HEV-IgG) ELISA Kit
yb-E11919 小鼠戊肝lgA（HEV-IgA）ELISA试剂盒 Mouse hepatitis E lgA (HEV-IgA) ELISA kit
yb-E11920 小鼠癌胚抗原（CEA）ELISA试剂盒 Mouse carcinoembryonic antigen (CEA) ELISA kit
yb-E11921 小鼠白细胞介素-6（IL-6）ELISA试剂盒 Mouse interleukin -6 (IL-6) ELISA Kit
yb-E11922 小鼠晚期糖基化终末产物 (AGEs)ELISA试剂盒 Sugar Mice advanced glycation end products (AGEs) ELISA kit
yb-E11923 小鼠胰岛素(Insulin)ELISA试剂盒 Mouse insulin (Insulin) ELISA Kit
yb-E11924 小鼠低密度脂蛋白（LDL)ELISA试剂盒 Mice low-density lipoprotein (LDL) ELISA kit
yb-E11925 小鼠高密度脂蛋白(HDL)ELISA试剂盒 Mice high-density lipoprotein (HDL) ELISA kit
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C-肽(CP)检测试剂盒
¥900 - 1890
