- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEB833Hu
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
补体因子D(CFD)检测试剂盒
检测范围 0.313-20ng/mL 灵敏度 0.125ng/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
补体因子D(CFD)检测试剂盒
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yb-E11013 人链激酶(SK)ELISA试剂盒 Human streptokinase (SK) ELISA Kit
yb-E11014 人核糖核酸酶(RNASE)ELISA试剂盒 Ribonuclease (RNASE) ELISA Kit
yb-E11015 人过氧化脂质/乳过氧化物酶(LPO)ELISA试剂盒 Human lipid peroxidation / lactoperoxidase (LPO) ELISA kit
yb-E11016 人芳基硫酸酯酶A(ASA)ELISA试剂盒 Human arylsulfatase A (ASA) ELISA Kit
yb-E11017 人对氧磷酶(PON)ELISA试剂盒 Human paraoxonase (PON) ELISA kit
yb-E11018 人丙酮酸激酶(PK)ELISA试剂盒 Human pyruvate kinase (PK) ELISA Kit
yb-E11019 人半乳糖6硫酸酯酶(Gal-6S)ELISA试剂盒 6 sulfatase people galactose (Gal-6S) ELISA kit
yb-E11020 人艾杜糖硫酸酯酶(IDS)ELISA试剂盒 Human iduronate sulfatase (IDS) ELISA Kit
yb-E11021 人β葡萄糖醛酸苷酶(βGD)ELISA试剂盒 Human β glucuronidase enzyme (βGD) ELISA kit
yb-E11022 人β葡糖苷酶(β-glucosidase)ELISA试剂盒 Human β -glucosidase (β-glucosidase) ELISA Kit
yb-E11023 人β内酰胺酶(β-lactamase)ELISA试剂盒 People in the β -lactamase (β-lactamase) ELISA kit
yb-E11024 人β甘露糖苷酶(β Manase)ELISA试剂盒 Human mannosidase β (β Manase) ELISA Kit
yb-E11025 人β半乳糖苷酶(βGAL)ELISA试剂盒 Human β -galactosidase (βGAL) ELISA Kit
yb-E11026 人αL艾杜糖苷酸酶(IDUA)ELISA试剂盒 People αL iduronidase (IDUA) ELISA kit
yb-E11027 人α2抗纤溶酶(α2-AP)ELISA试剂盒 Human α2 -antiplasmin (α2-AP) ELISA Kit
yb-E11028 人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒 Human N- acetyl -β-D- glucosaminidase (NAG) ELISA kit
yb-E11029 人L苯丙氨酸解氨酶(PAL)ELISA试剂盒 Human L phenylalanine ammonia lyase (PAL) ELISA kit
yb-E11030 人5核苷酸酶(5-NT)ELISA试剂盒 Person nucleotidase (5-NT) ELISA Kit
yb-E11031 人1,3-βD葡葡糖苷酶(1,3-βD-Glu)ELISA试剂盒 1,3-βD Portugal human glucosidase (1,3-βD-Glu) ELISA Kit
yb-E11032 人肌酸激酶(CK)ELISA试剂盒 Human creatine kinase (CK) ELISA kit
yb-E11033 人靶向核糖核酸酶(TR)ELISA试剂盒 Targeting human ribonuclease (TR) ELISA Kit
yb-E11034 人蛋白激酶C(PKC)ELISA试剂盒 Human protein kinase C (PKC) ELISA Kit
yb-E11035 人醛缩酶(ALD)ELISA试剂盒 Human aldolase (ALD) ELISA kit
yb-E11036 人L-乳酸脱氢酶(L-LDH)ELISA试剂盒 Human L- lactate dehydrogenase (L-LDH) ELISA Kit
补体因子D(CFD)检测试剂盒
检测范围 0.313-20ng/mL 灵敏度 0.125ng/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
补体因子D(CFD)检测试剂盒
ELISA Kit for Complement Factor D (CFD)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEB833Hu |
| Sample type | Serum, plasma and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.125ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Factor D (CFD).
No significant cross-reactivity or interference between Complement Factor D (CFD) and analogues was observed.
No significant cross-reactivity or interference between Complement Factor D (CFD) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Complement Factor D (CFD) and the recovery rates were calculated by comparing the measured value to the expected amount of Complement Factor D (CFD) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-98 | 94 |
| EDTA plasma(n=5) | 95-104 | 99 |
| heparin plasma(n=5) | 88-101 | 95 |
Precision
补体因子D(CFD)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor D (CFD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor D (CFD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor D (CFD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complement Factor D (CFD) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 93-105% | 80-89% | 79-92% |
| EDTA plasma(n=5) | 79-92% | 95-103% | 84-98% | 99-105% |
| heparin plasma(n=5) | 80-102% | 93-105% | 91-102% | 78-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
补体因子D(CFD)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D (CFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor D (CFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor D (CFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor D (CFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E11011 人磷酸葡萄糖变位酶(PGM)ELISA试剂盒 People phosphate mutase (PGM) ELISA kit
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yb-E11013 人链激酶(SK)ELISA试剂盒 Human streptokinase (SK) ELISA Kit
yb-E11014 人核糖核酸酶(RNASE)ELISA试剂盒 Ribonuclease (RNASE) ELISA Kit
yb-E11015 人过氧化脂质/乳过氧化物酶(LPO)ELISA试剂盒 Human lipid peroxidation / lactoperoxidase (LPO) ELISA kit
yb-E11016 人芳基硫酸酯酶A(ASA)ELISA试剂盒 Human arylsulfatase A (ASA) ELISA Kit
yb-E11017 人对氧磷酶(PON)ELISA试剂盒 Human paraoxonase (PON) ELISA kit
yb-E11018 人丙酮酸激酶(PK)ELISA试剂盒 Human pyruvate kinase (PK) ELISA Kit
yb-E11019 人半乳糖6硫酸酯酶(Gal-6S)ELISA试剂盒 6 sulfatase people galactose (Gal-6S) ELISA kit
yb-E11020 人艾杜糖硫酸酯酶(IDS)ELISA试剂盒 Human iduronate sulfatase (IDS) ELISA Kit
yb-E11021 人β葡萄糖醛酸苷酶(βGD)ELISA试剂盒 Human β glucuronidase enzyme (βGD) ELISA kit
yb-E11022 人β葡糖苷酶(β-glucosidase)ELISA试剂盒 Human β -glucosidase (β-glucosidase) ELISA Kit
yb-E11023 人β内酰胺酶(β-lactamase)ELISA试剂盒 People in the β -lactamase (β-lactamase) ELISA kit
yb-E11024 人β甘露糖苷酶(β Manase)ELISA试剂盒 Human mannosidase β (β Manase) ELISA Kit
yb-E11025 人β半乳糖苷酶(βGAL)ELISA试剂盒 Human β -galactosidase (βGAL) ELISA Kit
yb-E11026 人αL艾杜糖苷酸酶(IDUA)ELISA试剂盒 People αL iduronidase (IDUA) ELISA kit
yb-E11027 人α2抗纤溶酶(α2-AP)ELISA试剂盒 Human α2 -antiplasmin (α2-AP) ELISA Kit
yb-E11028 人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA试剂盒 Human N- acetyl -β-D- glucosaminidase (NAG) ELISA kit
yb-E11029 人L苯丙氨酸解氨酶(PAL)ELISA试剂盒 Human L phenylalanine ammonia lyase (PAL) ELISA kit
yb-E11030 人5核苷酸酶(5-NT)ELISA试剂盒 Person nucleotidase (5-NT) ELISA Kit
yb-E11031 人1,3-βD葡葡糖苷酶(1,3-βD-Glu)ELISA试剂盒 1,3-βD Portugal human glucosidase (1,3-βD-Glu) ELISA Kit
yb-E11032 人肌酸激酶(CK)ELISA试剂盒 Human creatine kinase (CK) ELISA kit
yb-E11033 人靶向核糖核酸酶(TR)ELISA试剂盒 Targeting human ribonuclease (TR) ELISA Kit
yb-E11034 人蛋白激酶C(PKC)ELISA试剂盒 Human protein kinase C (PKC) ELISA Kit
yb-E11035 人醛缩酶(ALD)ELISA试剂盒 Human aldolase (ALD) ELISA kit
yb-E11036 人L-乳酸脱氢酶(L-LDH)ELISA试剂盒 Human L- lactate dehydrogenase (L-LDH) ELISA Kit
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文献和实验相关实验
D 型氨基酸氧化酶（DAAO）与过氧化氢检测试剂盒联用，对产物中的 D 型氨基酸的总量进行评价。结果显示只有芽孢杆菌属和类芽孢杆菌属的肠道菌能够产生 D 型氨基酸，且在芽孢杆菌属内，菌株在种和菌株的水平上产 D 型氨基酸的能力也有差异，并与 16S rRNA 相似度没有明显的相关性（图 2），暗示单纯依靠 16S rRNA 测序获得微生物组菌种组成信息，无法准确预测菌群合成 D 型氨基酸的水平。 图 2：DAAO 法评估大鼠肠道菌生产 D 型氨基酸的能力与 16S rRNA 的关系 最后
一个试验真实性的资料归纳表 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 试 验 有病（真阳性） 无病（真阴性） 合 计 ──────────────────────────────── 阳 性 A B A+B ──────────────────────────────── 阴 性 C D C+D ──────────────────────────────── 总 数 A+C B+D A+B+C+D ━━━━━━━━━━━━━━━━━━━━━━━━━━
（Y 轴）。同时为了试验结果的直观性，图中提供的是原始数据而非对数值。推荐使用对数 值建立标准曲线图。由于实验操作条件的不同（如操作者、移液技术、洗板技术和温度条件等），标准曲线的 O.D. 值会有所差异。所提供的标准曲线仅供参考，实验者需要根据自已的实验建立标准曲线。大鼠白介素 12A （IL12A） 检测试剂盒标准曲线检测范围15.625pg/mL-1000pg/mL最低检测限6.1pg/mL此值为 20 个空白样品 （即标准品稀释液） 测定的平均值加二倍标准差所对应
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补体因子D(CFD)检测试剂盒
¥900 - 1890
