- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBED076Hu
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
碳酸酐酶Ⅸ(CA9)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 2.9pg/mL
样本类型 Serum, plasma, tissue homogenates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
碳酸酐酶Ⅸ(CA9)检测试剂盒
yb-E10986 人白细胞弹性蛋白酶(HLE)ELISA试剂盒 Human leukocyte elastase (HLE) ELISA Kit
yb-E10987 人弹性蛋白酶(Elastase)ELISA试剂盒 Human elastase (Elastase) ELISA Kit
yb-E10988 人α淀粉酶(AMS/AMY)ELISA试剂盒 Human α amylase (AMS / AMY) ELISA Kit
yb-E10989 人碱性磷酸酶(ALP)ELISA试剂盒 Human alkaline phosphatase (ALP) ELISA kit
yb-E10990 人天青杀素(AZU)ELISA试剂盒 Azure kill human hormone (AZU) ELISA Kit
yb-E10991 人血管紧张肽酶A(ATA)ELISA试剂盒 Human angiotensin enzyme A (ATA) ELISA kit
yb-E10992 人胰淀粉酶(PAMY)ELISA试剂盒 Human pancreatic amylase (PAMY) ELISA kit
yb-E10993 人α烯醇化酶(αENOL)ELISA试剂盒 Human α enolase (αENOL) ELISA kit
yb-E10994 人胶原酶II(Collagenase II)ELISA试剂盒 Human collagenase II (Collagenase II) ELISA Kit
yb-E10995 人胶原酶I(Collagenase I)ELISA试剂盒 Human collagenase I (Collagenase I) ELISA kit
yb-E10996 人磷酸化蛋白激酶C(P-PKC)ELISA试剂盒 Phosphorylation of human protein kinase C (P-PKC) ELISA Kit
yb-E10997 人乙酰胆碱酯酶(AChE)ELISA试剂盒 Human acetylcholinesterase (AChE) ELISA kit
yb-E10998 人葡萄糖氧化酶(GOD)ELISA试剂盒 Glucose oxidase person (GOD) ELISA Kit
yb-E10999 人酪氨酸羟化酶(TH)ELISA试剂盒 Human tyrosine hydroxylase (TH) ELISA kit
yb-E11000 人多巴胺脱羧酶(DDC)ELISA试剂盒 Human dopamine decarboxylase (DDC) ELISA Kit
yb-E11001 人非神经元性烯醇化酶(NNE)ELISA试剂盒 Human non- neuronal enolase (NNE) ELISA kit
yb-E11002 人多巴胺-β羟化酶(DBH)ELISA试剂盒 Human dopamine -β -hydroxylase (DBH) ELISA Kit
yb-E11003 人胆碱乙酰化酶(CHAc)ELISA试剂盒 Human choline acetyltransferase (CHAc) ELISA kit
yb-E11004 人异柠檬酸脱氢酶(ICD)ELISA试剂盒 People isocitrate dehydrogenase (ICD) ELISA kit
yb-E11005 人胰蛋白酶原Ⅱ(Try-Ⅱ)ELISA试剂盒 Human trypsinogen Ⅱ (Try-Ⅱ) ELISA kit
yb-E11006 人腺苷脱氨酶(ADA)ELISA试剂盒 Human adenosine deaminase (ADA) ELISA kit
yb-E11007 人透明质酸酶(HAase)ELISA试剂盒 Human hyaluronidase (HAase) ELISA Kit
yb-E11008 人天门冬氨酸氨基转移酶(AST)ELISA试剂盒 People aspartate aminotransferase (AST) ELISA kit
yb-E11009 人酸性磷酸酶(ACP)ELISA试剂盒 Human acid phosphatase (ACP) ELISA kit
碳酸酐酶Ⅸ(CA9)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 2.9pg/mL
样本类型 Serum, plasma, tissue homogenates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
碳酸酐酶Ⅸ(CA9)检测试剂盒
ELISA Kit for Carbonic Anhydrase IX (CA9)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SED076Hu |
| Sample type | Serum, plasma, tissue homogenates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 7.81-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL, 7.81pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 2.9pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase IX (CA9).
No significant cross-reactivity or interference between Carbonic Anhydrase IX (CA9) and analogues was observed.
No significant cross-reactivity or interference between Carbonic Anhydrase IX (CA9) and analogues was observed.
Recovery
碳酸酐酶Ⅸ(CA9)检测试剂盒Matrices listed below were spiked with certain level of recombinant Carbonic Anhydrase IX (CA9) and the recovery rates were calculated by comparing the measured value to the expected amount of Carbonic Anhydrase IX (CA9) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-103 | 96 |
| EDTA plasma(n=5) | 93-101 | 98 |
| heparin plasma(n=5) | 82-101 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase IX (CA9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase IX (CA9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase IX (CA9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carbonic Anhydrase IX (CA9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-97% | 98-105% | 90-97% | 78-95% |
| EDTA plasma(n=5) | 85-94% | 79-99% | 80-99% | 88-102% |
| heparin plasma(n=5) | 97-104% | 81-101% | 97-105% | 88-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
碳酸酐酶Ⅸ(CA9)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase IX (CA9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase IX (CA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase IX (CA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase IX (CA9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E10986 人白细胞弹性蛋白酶(HLE)ELISA试剂盒 Human leukocyte elastase (HLE) ELISA Kit
yb-E10987 人弹性蛋白酶(Elastase)ELISA试剂盒 Human elastase (Elastase) ELISA Kit
yb-E10988 人α淀粉酶(AMS/AMY)ELISA试剂盒 Human α amylase (AMS / AMY) ELISA Kit
yb-E10989 人碱性磷酸酶(ALP)ELISA试剂盒 Human alkaline phosphatase (ALP) ELISA kit
yb-E10990 人天青杀素(AZU)ELISA试剂盒 Azure kill human hormone (AZU) ELISA Kit
yb-E10991 人血管紧张肽酶A(ATA)ELISA试剂盒 Human angiotensin enzyme A (ATA) ELISA kit
yb-E10992 人胰淀粉酶(PAMY)ELISA试剂盒 Human pancreatic amylase (PAMY) ELISA kit
yb-E10993 人α烯醇化酶(αENOL)ELISA试剂盒 Human α enolase (αENOL) ELISA kit
yb-E10994 人胶原酶II(Collagenase II)ELISA试剂盒 Human collagenase II (Collagenase II) ELISA Kit
yb-E10995 人胶原酶I(Collagenase I)ELISA试剂盒 Human collagenase I (Collagenase I) ELISA kit
yb-E10996 人磷酸化蛋白激酶C(P-PKC)ELISA试剂盒 Phosphorylation of human protein kinase C (P-PKC) ELISA Kit
yb-E10997 人乙酰胆碱酯酶(AChE)ELISA试剂盒 Human acetylcholinesterase (AChE) ELISA kit
yb-E10998 人葡萄糖氧化酶(GOD)ELISA试剂盒 Glucose oxidase person (GOD) ELISA Kit
yb-E10999 人酪氨酸羟化酶(TH)ELISA试剂盒 Human tyrosine hydroxylase (TH) ELISA kit
yb-E11000 人多巴胺脱羧酶(DDC)ELISA试剂盒 Human dopamine decarboxylase (DDC) ELISA Kit
yb-E11001 人非神经元性烯醇化酶(NNE)ELISA试剂盒 Human non- neuronal enolase (NNE) ELISA kit
yb-E11002 人多巴胺-β羟化酶(DBH)ELISA试剂盒 Human dopamine -β -hydroxylase (DBH) ELISA Kit
yb-E11003 人胆碱乙酰化酶(CHAc)ELISA试剂盒 Human choline acetyltransferase (CHAc) ELISA kit
yb-E11004 人异柠檬酸脱氢酶(ICD)ELISA试剂盒 People isocitrate dehydrogenase (ICD) ELISA kit
yb-E11005 人胰蛋白酶原Ⅱ(Try-Ⅱ)ELISA试剂盒 Human trypsinogen Ⅱ (Try-Ⅱ) ELISA kit
yb-E11006 人腺苷脱氨酶(ADA)ELISA试剂盒 Human adenosine deaminase (ADA) ELISA kit
yb-E11007 人透明质酸酶(HAase)ELISA试剂盒 Human hyaluronidase (HAase) ELISA Kit
yb-E11008 人天门冬氨酸氨基转移酶(AST)ELISA试剂盒 People aspartate aminotransferase (AST) ELISA kit
yb-E11009 人酸性磷酸酶(ACP)ELISA试剂盒 Human acid phosphatase (ACP) ELISA kit
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碳酸酐酶Ⅸ(CA9)检测试剂盒
¥900 - 1890
