适用生物 Homo sapiens (Human,人)
巨噬细胞集落刺激因子(MCSF)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 3.2pg/mL
样本类型 Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
巨噬细胞集落刺激因子(MCSF)检测试剂盒
ELISA Kit for Colony Stimulating Factor 1, Macrophage (MCSF)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species |
Homo sapiens (Human) |
Product No. |
SEA090Hu |
Sample type |
Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Format |
96T |
Assay length |
4.5 hours |
Detection range |
7.81-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL, 7.81pg/mL |
Sensitivity |
The minimum detectable dose of this kit is typically less than 3.2pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 1, Macrophage (MCSF).
No significant cross-reactivity or interference between Colony Stimulating Factor 1, Macrophage (MCSF) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Colony Stimulating Factor 1, Macrophage (MCSF) and the recovery rates were calculated by comparing the measured value to the expected amount of Colony Stimulating Factor 1, Macrophage (MCSF) in samples.
Matrix |
Recovery range (%) |
Average(%) |
serum(n=5) |
78-101 |
94 |
EDTA plasma(n=5) |
96-103 |
101 |
heparin plasma(n=5) |
78-94 |
88 |
Precision
巨噬细胞集落刺激因子(MCSF)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 1, Macrophage (MCSF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 1, Macrophage (MCSF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Colony Stimulating Factor 1, Macrophage (MCSF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
88-104% |
82-96% |
80-96% |
78-101% |
EDTA plasma(n=5) |
99-105% |
89-98% |
79-97% |
93-102% |
heparin plasma(n=5) |
80-104% |
80-102% |
99-105% |
89-98% |
Stability
巨噬细胞集落刺激因子(MCSF)检测试剂盒The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents |
Quantity |
Reagents |
Quantity |
Pre-coated, ready to use 96-well strip plate |
1 |
Plate sealer for 96 wells |
4 |
Standard |
2 |
Standard Diluent |
1×20mL |
Detection Reagent A |
1×120µL |
Assay Diluent A |
1×12mL |
Detection Reagent B |
1×120µL |
Assay Diluent B |
1×12mL |
TMB Substrate |
1×9mL |
Stop Solution |
1×6mL |
Wash Buffer (30 × concentrate) |
1×20mL |
Instruction manual |
1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 1, Macrophage (MCSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 1, Macrophage (MCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 1, Macrophage (MCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 1, Macrophage (MCSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E10396 人内皮型一氧化氮合成酶3(eNOS-3)ELISA试剂盒 Human endothelial nitric oxide synthase 3 (eNOS-3) ELISA kit
yb-E10397 人丙二醛(MDA)ELISA试剂盒 People malondialdehyde (MDA) ELISA kit
yb-E10398 人胰淀素(Amylin)ELISA试剂盒 Human amylin (Amylin) ELISA kit
yb-E10399 人血管活性肠肽(VIP)ELISA试剂盒 Human vasoactive intestinal peptide (VIP) ELISA kit
yb-E10400 人胆囊收缩素/肠促胰酶肽(CCK)ELISA试剂盒 Human cholecystokinin / incretin trypsin peptides (CCK) ELISA kit
yb-E10401 人Ⅲ型前胶原肽(PⅢNP)ELISA试剂盒 People Ⅲ procollagen peptide (P Ⅲ NP) ELISA kit
yb-E10402 人Ⅱ型胶原(Col Ⅱ)ELISA试剂盒 People Ⅱ collagen (Col Ⅱ) ELISA kit
yb-E10403 人Ⅰ型胶原(Col Ⅰ)ELISA试剂盒 Human collagen type Ⅰ (Col Ⅰ) ELISA kit
yb-E10404 人Ⅰ型前胶原羧基端肽(PⅠCP)ELISA试剂盒 People Ⅰ procollagen carboxy-terminal peptide (P Ⅰ CP) ELISA kit
yb-E10405 人Ⅳ型胶原(Col Ⅳ)ELISA试剂盒 Human collagen type Ⅳ (Col Ⅳ) ELISA kit
yb-E10406 人Ⅲ型胶原(Col Ⅲ)ELISA试剂盒 People Ⅲ collagen (Col Ⅲ) ELISA kit
yb-E10407 人层连蛋白/板层素(LN)ELISA试剂盒 Human laminin / laminin (LN) ELISA kit
yb-E10408 人纤连蛋白(FN)ELISA试剂盒 Human fibronectin (FN) ELISA kit
yb-E10409 人NOGO-A抗体(Nogo-A Ab)ELISA试剂盒 Human NOGO-A antibody (Nogo-A Ab) ELISA Kit
yb-E10410 人抗组织转谷氨酰胺酶抗体IgA(tTG-IgA)ELISA试剂盒 Human anti- tissue transglutaminase antibody IgA (tTG-IgA) ELISA kit
yb-E10411 人抗存活素抗体/生存蛋白(Surv)ELISA试剂盒 Human anti- survivin antibody / survival protein (Surv) ELISA kit
yb-E10412 人粒细胞巨噬细胞集落刺激因子抗体(GM-CSF Ab)ELISA试剂盒 Human granulocyte- macrophage colony stimulating factor antibody cell (GM-CSF Ab) ELISA kit
yb-E10413 人抗肌联蛋白抗体(TTN)ELISA试剂盒 Human anti- titin antibody (TTN) ELISA Kit
yb-E10414 人抗突触前膜抗体(PsmAb)ELISA试剂盒 Human anti- presynaptic membrane antibody (PsmAb) ELISA kit
yb-E10415 人血管紧张素Ⅱ受体2抗体(AT2R-Ab)ELISA试剂盒 Angiotensin Ⅱ receptor 2 antibody (AT2R-Ab) ELISA kit
yb-E10416 人血管紧张素Ⅱ受体1抗体(ATⅡR1)ELISA试剂盒 Angiotensin Ⅱ receptor antibody (AT Ⅱ R1) ELISA kit
yb-E10417 人血管紧张素Ⅰ受体抗体(ANG-ⅠR)ELISA试剂盒 Angiotensin Ⅰ receptor antibody (ANG-Ⅰ R) ELISA kit