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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
to free 3’-OH DNA ends in a template-independent manner, is used to label DNA strand breaks. Incorporated nucleotides are detected by a secondary antibody, conjugated with peroxidase. After substrate reaction, stained cells can be detected under light
as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance
ELISA PROTOCOL（Yale University）
antibody conjugated to AlkalinePhosphatase. I use a 1:500 dilution of rabbit anti-mouse IgG(Zymed)。 Incubate at room temperature for 1 hour. About 15 minbefore the end of the incubation start dissolving the p-nitrophenylphosphate substrate tablets
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