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- 钰博生物
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- 详细信息
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
白介素15(IL15)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 6.1pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
白介素15(IL15)检测试剂盒
yb-E10705 人降钙素基因相关肽(CGRP)ELISA试剂盒 Human calcitonin gene-related peptide (CGRP) ELISA kit
yb-E10706 人生长抑素(SS)ELISA试剂盒 Human somatostatin (SS) ELISA Kit
yb-E10707 人生长激素释放多肽(GHRP) ELISA试剂盒 Human growth hormone releasing peptide (GHRP) ELISA Kit
yb-E10708 人抑制素B(INH-B)ELISA试剂盒 Inhibin B (INH-B) ELISA kit
yb-E10709 人促甲状腺素释放激素(TRH)ELISA试剂盒 Human thyrotropin-releasing hormone (TRH) ELISA kit
yb-E10710 人褪黑素(MT)ELISA试剂盒 Human melatonin (MT) ELISA Kit
yb-E10711 人6酮前列腺素(6-K-PG)ELISA试剂盒 People 6 keto prostaglandin (6-K-PG) ELISA kit
yb-E10712 人血栓素B2(TXB2)ELISA试剂盒 Human thromboxane B2 (TXB2) ELISA kit
yb-E10713 人皮质酮/肾上腺酮(CORT)ELISA试剂盒 People corticosterone / corticosterone (CORT) ELISA kit
yb-E10714 人前列腺素E2(PGE2)ELISA试剂盒 Human prostaglandin E2 (PGE2) ELISA kit
yb-E10715 人双氢睾酮(DHT)ELISA试剂盒 People dihydrotestosterone (DHT) ELISA kit
yb-E10716 人促生长激素释放激素(GHRH)ELISA试剂盒 Human growth hormone releasing hormone (GHRH) ELISA kit
yb-E10717 人雌激素(E)ELISA试剂盒 Human estrogen (E) ELISA Kit
yb-E10718 人孕激素/孕酮(PROG)ELISA试剂盒 People progestin / progesterone (PROG) ELISA kit
yb-E10719 人超敏游离雌三醇(uE3)ELISA试剂盒 Hypersensitive people unconjugated estriol (uE3) ELISA kit
yb-E10720 人去甲肾上腺素(NA) ELISA试剂盒 People noradrenaline (NA) ELISA kit
yb-E10721 人前列腺素F(PGF)ELISA试剂盒 Human prostaglandin F (PGF) ELISA Kit
yb-E10722 人前列腺素E1(PGE1)ELISA试剂盒 Human prostaglandin E1 (PGE1) ELISA kit
yb-E10723 人内皮素1(ET-1)ELISA试剂盒 Human endothelin- 1 (ET-1) ELISA Kit
yb-E10724 人促卵泡素(FSH)ELISA试剂盒 Human follicle stimulating hormone (FSH) ELISA kit
yb-E10725 人游离β绒毛膜促性腺激素(f-βhCG)ELISA试剂盒 Free human chorionic gonadotropin β (f-βhCG) ELISA Kit
yb-E10726 人大内皮素(Big ET)ELISA试剂盒 NPC endothelin (Big ET) ELISA kit
yb-E10727 人全段甲状旁腺素(i-PTH)ELISA试剂盒 Human parathyroid hormone (i-PTH) ELISA kit
yb-E10728 人降钙素(CT)ELISA试剂盒 Human calcitonin (CT) ELISA kit
白介素15(IL15)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 6.1pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
白介素15(IL15)检测试剂盒
ELISA Kit for Interleukin 15 (IL15)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA061Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 6.1pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 15 (IL15).
No significant cross-reactivity or interference between Interleukin 15 (IL15) and analogues was observed.
No significant cross-reactivity or interference between Interleukin 15 (IL15) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 15 (IL15) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 15 (IL15) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-102 | 97 |
| EDTA plasma(n=5) | 89-97 | 92 |
| heparin plasma(n=5) | 82-91 | 88 |
Precision
白介素15(IL15)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 15 (IL15) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 15 (IL15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 15 (IL15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 15 (IL15) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-96% | 89-96% | 94-105% | 90-103% |
| EDTA plasma(n=5) | 93-105% | 91-99% | 95-102% | 90-104% |
| heparin plasma(n=5) | 80-104% | 78-94% | 90-99% | 79-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
白介素15(IL15)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 15 (IL15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 15 (IL15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 15 (IL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 15 (IL15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E10705 人降钙素基因相关肽(CGRP)ELISA试剂盒 Human calcitonin gene-related peptide (CGRP) ELISA kit
yb-E10706 人生长抑素(SS)ELISA试剂盒 Human somatostatin (SS) ELISA Kit
yb-E10707 人生长激素释放多肽(GHRP) ELISA试剂盒 Human growth hormone releasing peptide (GHRP) ELISA Kit
yb-E10708 人抑制素B(INH-B)ELISA试剂盒 Inhibin B (INH-B) ELISA kit
yb-E10709 人促甲状腺素释放激素(TRH)ELISA试剂盒 Human thyrotropin-releasing hormone (TRH) ELISA kit
yb-E10710 人褪黑素(MT)ELISA试剂盒 Human melatonin (MT) ELISA Kit
yb-E10711 人6酮前列腺素(6-K-PG)ELISA试剂盒 People 6 keto prostaglandin (6-K-PG) ELISA kit
yb-E10712 人血栓素B2(TXB2)ELISA试剂盒 Human thromboxane B2 (TXB2) ELISA kit
yb-E10713 人皮质酮/肾上腺酮(CORT)ELISA试剂盒 People corticosterone / corticosterone (CORT) ELISA kit
yb-E10714 人前列腺素E2(PGE2)ELISA试剂盒 Human prostaglandin E2 (PGE2) ELISA kit
yb-E10715 人双氢睾酮(DHT)ELISA试剂盒 People dihydrotestosterone (DHT) ELISA kit
yb-E10716 人促生长激素释放激素(GHRH)ELISA试剂盒 Human growth hormone releasing hormone (GHRH) ELISA kit
yb-E10717 人雌激素(E)ELISA试剂盒 Human estrogen (E) ELISA Kit
yb-E10718 人孕激素/孕酮(PROG)ELISA试剂盒 People progestin / progesterone (PROG) ELISA kit
yb-E10719 人超敏游离雌三醇(uE3)ELISA试剂盒 Hypersensitive people unconjugated estriol (uE3) ELISA kit
yb-E10720 人去甲肾上腺素(NA) ELISA试剂盒 People noradrenaline (NA) ELISA kit
yb-E10721 人前列腺素F(PGF)ELISA试剂盒 Human prostaglandin F (PGF) ELISA Kit
yb-E10722 人前列腺素E1(PGE1)ELISA试剂盒 Human prostaglandin E1 (PGE1) ELISA kit
yb-E10723 人内皮素1(ET-1)ELISA试剂盒 Human endothelin- 1 (ET-1) ELISA Kit
yb-E10724 人促卵泡素(FSH)ELISA试剂盒 Human follicle stimulating hormone (FSH) ELISA kit
yb-E10725 人游离β绒毛膜促性腺激素(f-βhCG)ELISA试剂盒 Free human chorionic gonadotropin β (f-βhCG) ELISA Kit
yb-E10726 人大内皮素(Big ET)ELISA试剂盒 NPC endothelin (Big ET) ELISA kit
yb-E10727 人全段甲状旁腺素(i-PTH)ELISA试剂盒 Human parathyroid hormone (i-PTH) ELISA kit
yb-E10728 人降钙素(CT)ELISA试剂盒 Human calcitonin (CT) ELISA kit
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白介素15(IL15)检测试剂盒
¥900 - 1890
