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- 钰博生物
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- YBEA547Hu
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- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
血管细胞粘附分子1(VCAM1)检测试剂盒
检测范围 0.781-50ng/mL 灵敏度 0.35ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
血管细胞粘附分子1(VCAM1)检测试剂盒
yb-E10472 人抗神经元核抗体1型/抗Hu抗体(ANNA-1/Hu)ELISA试剂盒 Human anti-neuronal nuclear antibody type 1 / anti- Hu antibodies (ANNA-1/Hu) ELISA kit
yb-E10473 人抗DNA酶B抗体(anti-DNase B)ELISA试剂盒 Human anti- DNA antibody enzyme B (anti-DNase B) ELISA kit
yb-E10474 人抗BP180抗体(BP180-Ab)ELISA试剂盒 Anti- human antibody BP180 (BP180-Ab) ELISA Kit
yb-E10475 人抗BB抗体(BB-Ab)ELISA试剂盒 Anti- human antibody BB (BB-Ab) ELISA Kit
yb-E10476 人抗平滑肌抗体(ASMA)ELISA试剂盒 Human anti- smooth muscle antibodies (ASMA) ELISA kit
yb-E10477 人抗肝细胞膜抗体(LMA)ELISA试剂盒 Human anti- liver cell membrane antibody (LMA) ELISA kit
yb-E10478 人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒 Human antibodies against liver -specific lipoprotein (LSP) ELISA kit
yb-E10479 人抗眼肌抗体(EMAb)ELISA试剂盒 Human anti- muscle antibody (EMAb) ELISA Kit
yb-E10480 人抗补体1q抗体(C1q)ELISA试剂盒 Anti- human antibody complement 1q (C1q) ELISA Kit
yb-E10481 人抗内因子抗体(IFA)ELISA试剂盒 The human anti- factor antibody (IFA) ELISA kit
yb-E10482 人抗磷脂抗体(Apl/APA)ELISA试剂盒 Human antiphospholipid antibodies (Apl / APA) ELISA kit
yb-E10483 人抗组蛋白抗体(AHA)ELISA试剂盒 Human anti- histone antibody (AHA) ELISA Kit
yb-E10484 人晶体蛋白α(Cryα)ELISA试剂盒 One crystal protein α (Cryα) ELISA kit
yb-E10485 人晶体蛋白β(Cryβ)ELISA试剂盒 One crystal protein β (Cryβ) ELISA kit
yb-E10486 人抗胶原蛋白抗体(CLA)ELISA试剂盒 Human anti -collagen antibodies (CLA) ELISA Kit
yb-E10487 人抗角蛋白丝聚集素/丝集蛋白抗体(AFA)ELISA试剂盒 Human anti- keratin filaments gathered hormone / wire assembly protein antibody (AFA) ELISA kit
yb-E10488 人抗环胍氨酸肽抗体(CCP)ELISA试剂盒 Human anti- cyclic citrulline peptide antibody (CCP) ELISA kit
yb-E10489 人抗核周因子抗体(APF)ELISA试剂盒 Human anti- perinuclear factor antibodies (APF) ELISA kit
yb-E10490 人抗核抗体(ANA)ELISA试剂盒 Human anti -nuclear antibody (ANA) ELISA kit
yb-E10491 人抗肝肾微粒体抗体(LKM)ELISA试剂盒 Human anti- kidney microsomal antibodies (LKM) ELISA kit
yb-E10492 人抗α干扰素抗体(IFNα-Ab)ELISA试剂盒 Anti- human interferon- α antibody (IFNα-Ab) ELISA Kit
yb-E10493 人抗Sc1-70抗体(Sc1-70-Ab)ELISA试剂盒 Sc1-70 anti- human antibody (Sc1-70-Ab) ELISA Kit
血管细胞粘附分子1(VCAM1)检测试剂盒
检测范围 0.781-50ng/mL 灵敏度 0.35ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
血管细胞粘附分子1(VCAM1)检测试剂盒
ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA547Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.781-50ng/mL The standard curve concentrations used for the ELISA’s were 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL, 0.781ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.35ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1).
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Vascular Cell Adhesion Molecule 1 (VCAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Cell Adhesion Molecule 1 (VCAM1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 85-99 | 95 |
| EDTA plasma(n=5) | 80-103 | 92 |
| heparin plasma(n=5) | 97-104 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-91% | 95-103% | 81-93% | 79-90% |
| EDTA plasma(n=5) | 98-105% | 81-105% | 99-105% | 95-102% |
| heparin plasma(n=5) | 85-96% | 78-105% | 80-103% | 93-103% |
Stability
血管细胞粘附分子1(VCAM1)检测试剂盒The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
血管细胞粘附分子1(VCAM1)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E10472 人抗神经元核抗体1型/抗Hu抗体(ANNA-1/Hu)ELISA试剂盒 Human anti-neuronal nuclear antibody type 1 / anti- Hu antibodies (ANNA-1/Hu) ELISA kit
yb-E10473 人抗DNA酶B抗体(anti-DNase B)ELISA试剂盒 Human anti- DNA antibody enzyme B (anti-DNase B) ELISA kit
yb-E10474 人抗BP180抗体(BP180-Ab)ELISA试剂盒 Anti- human antibody BP180 (BP180-Ab) ELISA Kit
yb-E10475 人抗BB抗体(BB-Ab)ELISA试剂盒 Anti- human antibody BB (BB-Ab) ELISA Kit
yb-E10476 人抗平滑肌抗体(ASMA)ELISA试剂盒 Human anti- smooth muscle antibodies (ASMA) ELISA kit
yb-E10477 人抗肝细胞膜抗体(LMA)ELISA试剂盒 Human anti- liver cell membrane antibody (LMA) ELISA kit
yb-E10478 人抗肝特异性脂蛋白抗体(LSP)ELISA试剂盒 Human antibodies against liver -specific lipoprotein (LSP) ELISA kit
yb-E10479 人抗眼肌抗体(EMAb)ELISA试剂盒 Human anti- muscle antibody (EMAb) ELISA Kit
yb-E10480 人抗补体1q抗体(C1q)ELISA试剂盒 Anti- human antibody complement 1q (C1q) ELISA Kit
yb-E10481 人抗内因子抗体(IFA)ELISA试剂盒 The human anti- factor antibody (IFA) ELISA kit
yb-E10482 人抗磷脂抗体(Apl/APA)ELISA试剂盒 Human antiphospholipid antibodies (Apl / APA) ELISA kit
yb-E10483 人抗组蛋白抗体(AHA)ELISA试剂盒 Human anti- histone antibody (AHA) ELISA Kit
yb-E10484 人晶体蛋白α(Cryα)ELISA试剂盒 One crystal protein α (Cryα) ELISA kit
yb-E10485 人晶体蛋白β(Cryβ)ELISA试剂盒 One crystal protein β (Cryβ) ELISA kit
yb-E10486 人抗胶原蛋白抗体(CLA)ELISA试剂盒 Human anti -collagen antibodies (CLA) ELISA Kit
yb-E10487 人抗角蛋白丝聚集素/丝集蛋白抗体(AFA)ELISA试剂盒 Human anti- keratin filaments gathered hormone / wire assembly protein antibody (AFA) ELISA kit
yb-E10488 人抗环胍氨酸肽抗体(CCP)ELISA试剂盒 Human anti- cyclic citrulline peptide antibody (CCP) ELISA kit
yb-E10489 人抗核周因子抗体(APF)ELISA试剂盒 Human anti- perinuclear factor antibodies (APF) ELISA kit
yb-E10490 人抗核抗体(ANA)ELISA试剂盒 Human anti -nuclear antibody (ANA) ELISA kit
yb-E10491 人抗肝肾微粒体抗体(LKM)ELISA试剂盒 Human anti- kidney microsomal antibodies (LKM) ELISA kit
yb-E10492 人抗α干扰素抗体(IFNα-Ab)ELISA试剂盒 Anti- human interferon- α antibody (IFNα-Ab) ELISA Kit
yb-E10493 人抗Sc1-70抗体(Sc1-70-Ab)ELISA试剂盒 Sc1-70 anti- human antibody (Sc1-70-Ab) ELISA Kit
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血管细胞粘附分子1(VCAM1)检测试剂盒
¥900 - 1890
