- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA045Ra
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Rattus norvegicus (Rat，大鼠)
粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 3.2pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒
yb-E13480 鱼磷酸化腺苷酸活化蛋白激酶ELISA试剂盒 Fish AMP-activated protein kinase phosphorylation
yb-E13481 鱼卵黄高磷蛋白ELISA试剂盒 Yellow roe high phosphoprotein
yb-E13482 鱼类主要组织相容性复合体ELISA试剂盒 Fish major histocompatibility complex
yb-E13483 鲑鱼补体蛋白3ELISA试剂盒 Salmon complement protein 3
yb-E13484 鲑鱼补体蛋白4ELISA试剂盒 Salmon complement protein 4
yb-E13485 鱼类白介素1αELISA试剂盒 Fish interleukin- 1α
yb-E13486 鱼类白介素1βELISA试剂盒 Fish interleukin- 1β
yb-E13487 鱼三碘甲状腺原氨酸(T3)ELISA试剂盒 Fish three triiodothyronine (T3) ELISA kit
yb-E13488 鱼甲状腺素(T4)ELISA试剂盒 Fish thyroxine (T4) ELISA kit
yb-E13489 鱼总补体(CH50)ELISA试剂盒 Fish total complement (CH50) ELISA kit
yb-E13490 鲤鱼卵黄蛋白原ELISA试剂盒 Carp vitellogenin
yb-E13491 鱼类促性腺激素释放激素ELISA试剂盒 Fish gonadotropin-releasing hormone
yb-E13492 鱼类三碘甲状腺原氨酸(T3)ELISA试剂盒 Fish three triiodothyronine (T3) ELISA kit
yb-E13493 鱼类皮质醇(Cortisol)ELISA试剂盒 Fish cortisol (Cortisol) ELISA kit
yb-E13494 鱼类超敏C反应蛋白(hs-CRP)ELISA试剂盒 Fish high-sensitivity C -reactive protein (hs-CRP) ELISA kit
yb-E13495 鱼骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒 Fish osteocalcin / bone glutamic acid protein (OT / BGP) ELISA kit
yb-E13496 红螯光壳螯虾卵黄脂磷蛋白/卵黄磷蛋白(Lv/Vn)ELISA试剂盒 Redclaw light shell crawfish lipovitellin / Vitellin (Lv / Vn) ELISA kit
yb-E13497 罗氏沼虾诺达病毒(MrNV)ELISA试剂盒 Rosenbergii nodavirus (MrNV) ELISA kit
yb-E13498 毒蛇因子(CVF)ELISA试剂盒 Viper factor (CVF) ELISA Kit
yb-E13499 牛蛙生长激素（GH）ELISA试剂盒 Bullfrog growth hormone (GH) ELISA kit
yb-E13500 牛结核分枝杆菌抗体（M.tuberculosis Ab）ELISA试剂盒 Mycobacterium bovis antibody (M.tuberculosis Ab) ELISA kit
粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 3.2pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒
ELISA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | SEA045Ra |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 7.81-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL, 7.81pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 3.2pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF).
No significant cross-reactivity or interference between Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and analogues was observed.
No significant cross-reactivity or interference between Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and analogues was observed.
Recovery
粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒Matrices listed below were spiked with certain level of recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and the recovery rates were calculated by comparing the measured value to the expected amount of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-104 | 90 |
| EDTA plasma(n=5) | 79-101 | 95 |
| heparin plasma(n=5) | 96-105 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-90% | 97-105% | 83-103% | 94-103% |
| EDTA plasma(n=5) | 80-102% | 87-95% | 90-99% | 98-105% |
| heparin plasma(n=5) | 85-95% | 86-97% | 79-96% | 90-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E13480 鱼磷酸化腺苷酸活化蛋白激酶ELISA试剂盒 Fish AMP-activated protein kinase phosphorylation
yb-E13481 鱼卵黄高磷蛋白ELISA试剂盒 Yellow roe high phosphoprotein
yb-E13482 鱼类主要组织相容性复合体ELISA试剂盒 Fish major histocompatibility complex
yb-E13483 鲑鱼补体蛋白3ELISA试剂盒 Salmon complement protein 3
yb-E13484 鲑鱼补体蛋白4ELISA试剂盒 Salmon complement protein 4
yb-E13485 鱼类白介素1αELISA试剂盒 Fish interleukin- 1α
yb-E13486 鱼类白介素1βELISA试剂盒 Fish interleukin- 1β
yb-E13487 鱼三碘甲状腺原氨酸(T3)ELISA试剂盒 Fish three triiodothyronine (T3) ELISA kit
yb-E13488 鱼甲状腺素(T4)ELISA试剂盒 Fish thyroxine (T4) ELISA kit
yb-E13489 鱼总补体(CH50)ELISA试剂盒 Fish total complement (CH50) ELISA kit
yb-E13490 鲤鱼卵黄蛋白原ELISA试剂盒 Carp vitellogenin
yb-E13491 鱼类促性腺激素释放激素ELISA试剂盒 Fish gonadotropin-releasing hormone
yb-E13492 鱼类三碘甲状腺原氨酸(T3)ELISA试剂盒 Fish three triiodothyronine (T3) ELISA kit
yb-E13493 鱼类皮质醇(Cortisol)ELISA试剂盒 Fish cortisol (Cortisol) ELISA kit
yb-E13494 鱼类超敏C反应蛋白(hs-CRP)ELISA试剂盒 Fish high-sensitivity C -reactive protein (hs-CRP) ELISA kit
yb-E13495 鱼骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒 Fish osteocalcin / bone glutamic acid protein (OT / BGP) ELISA kit
yb-E13496 红螯光壳螯虾卵黄脂磷蛋白/卵黄磷蛋白(Lv/Vn)ELISA试剂盒 Redclaw light shell crawfish lipovitellin / Vitellin (Lv / Vn) ELISA kit
yb-E13497 罗氏沼虾诺达病毒(MrNV)ELISA试剂盒 Rosenbergii nodavirus (MrNV) ELISA kit
yb-E13498 毒蛇因子(CVF)ELISA试剂盒 Viper factor (CVF) ELISA Kit
yb-E13499 牛蛙生长激素（GH）ELISA试剂盒 Bullfrog growth hormone (GH) ELISA kit
yb-E13500 牛结核分枝杆菌抗体（M.tuberculosis Ab）ELISA试剂盒 Mycobacterium bovis antibody (M.tuberculosis Ab) ELISA kit
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文献和实验相关实验
Expression on Eosinophils by p38 Mitogen-Activated Protein Kinase and Nuclear Factor-κB 白细胞介素-3,-5，和粒巨噬细胞集落刺激因子（GMCSF）通过p38丝裂原活化蛋白激酶（MAPK）和核转录因子NFκB诱导嗜酸性粒细胞中黏附分子的表达 嗜酸性粒细胞是一种重要的炎症效应细胞，它通常集聚在变应性炎症部位如气道粘膜，被激活的嗜酸性粒细胞释放细胞毒性分子。嗜酸性粒细胞的细胞膜上表达有多种黏附分子如免疫球蛋白（Ig）超家族
树突状细胞来做为瘤苗。(5)与细胞因子联合使用:ＩＬ12是体内介导ＴＨ1型应答的细胞因子,与抗原冲击的树突状细胞瘤苗联合应用于肿瘤治疗,收到更佳的抗癌效果。某些情况下甚至逆转由树突状细胞诱导的耐受。肿瘤相关抗原和肿瘤特异性抗原:粒细胞、巨噬细胞集落刺激因子(ＧＭＣＳＦ)可以促进树突状细胞的成熟,维持树突状细胞的存活。 将ＧＭＣＳＦ基因经腺病毒载体介导转入树突状细胞,具有更强的体内激发肿瘤特异性ＣＴＬ的能力。用腺病毒将ＩＬ12基因转染树突状细胞,也可协同增强Ｔ细胞功能,促进机体的抗肿瘤效应。Ｆｍｓ
应该比任何方法都有效，因为每一步都切中要害，每一步周到得滴水不漏。 4、不含GM-CSF和IL-4的培养基先培养数小时？ 我们都知道GM-CSF是诱导DC分化、生长和发育的。那么为什么一开始要用不含GM-CSF培养3 小时， 甚至几十小时后，再加GM-CSF呢？这样骨髓细胞已经向其他方向分化了，难道再用GM-CSF可以把粒细胞或巨噬细胞“逆分化”成DC吗？有如：我们在十字路口明明是要向右转，却一定要先向左转开一段路，再180度掉头向后转呢？就如我在第1点中说的，非要把DC细胞折腾到濒临
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粒细胞巨噬细胞集落刺激因子(GMCSF)检测试剂盒
¥900 - 1890
