Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls red blood cell production (erythropoiesis). It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. The EPO gene encodes a deduced 193-amino acid propolypeptide hormone that can be found in the kidney and liver. This hormone regulates red blood cell production, plays an important role in the brain's response to neuronal injury, and is involved in the wound healing process. ScienCell's mouse EPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse EPO species-specific polyclonal antibodies are precoated onto 8 x 12 divisible strips. The mouse specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added, and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse EPO sample that is captured in strips.
Catalog No.
EK0333
Country of Manufacture
United States
Product Code
mEPO-ELISA
Size/Quantity
96 tests
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.
Shipping Info
Shipped on gel ice.
References
1. Siren AL et al. (2001). "Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress". Proc Natl Acad Sci USA 98 (7): 4044-4049. doi:10.1073/pnas.051606598. PMC 31176. PMID 11259643. 2. Haroon ZA, Aminutes K, Jiang X, Arcasoy MO (September 2003). "A novel role for erythropoietin during fibrin-induced wound-healing response". Am. J. Pathol. 163 (3): 993-1000. PMC 1868246. PMID 12937140. 3. Brines, M. L., Ghezzi, P., Keenan, S., Agnello, D., de Lanerolle, N. C., Cerami, C., Itri, L. M., Cerami, A. Erythropoietin crosses the blood-brain barrier to protect against experimental brain injury. Proc. Nat. Acad. Sci. 97: 10526-10531, 2000. [PubMed: 10984541, related citations] [Full Text: HighWire Press, Pubget]