Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. ScienCell's rat IL-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat IL-2-specific monoclonal antibodies are precoated onto 8 x 12 strips. The rat specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat IL-2 in the sample that is captured in the strips.
Catalog No.
EK0406
Country of Manufacture
United States
Product Code
rIL-2 ?ELISA
Size/Quantity
96 tests
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.
Shipping Info
Shipped on gel ice.
References
1. Prokopchuk, O.; Liu, Y.; Henne-Bruns, D.; Kornmann, M. Interleukin-4 enhances proliferation of human pancreatic cancer cells: evidence for autocrine and paracrine actions. Br J Cancer.2005 Mar 14;92(5):921-8. 2. Arai, N.; Nomura, D.; Villaret, D.; DeWaal Malefijt, R.; Seiki, M.; Yoshida, M.; Minoshima, S.; Fukuyama, R.; Maekawa, M.; Kudoh, J.; Shimizu, N.; Yokota, K.; Abe, E.; Yokota, T.; Takebe, Y.; Arai, K. Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression. J. Immun. 142: 274-282, 1989.