Description |
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Monoclonal Antibody against RNA Polymerase II |
Intended Use |
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**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
Clone |
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H5 |
Form |
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Ascites |
Host |
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RNA Polymerase II H5 Monoclonal Antibody Mouse |
Species Reactivity |
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Extensive (Yeast to Human) |
IsoType |
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IgM |
[Ab] |
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2-3 mg/mL |
Specificity |
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The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 2 version of pol II. |
Uses |
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This antibody is effective in immunofluorescence (IF), immunoblotting (WB), immunoprecipitation (IP), pol II purification and functional studies. |
Suggested Working Dilution |
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Quality control tests were done by Western blot and Dot blot. The optimal working dilution should be determined for each specific assay condition.
- WB: 1:500*
- IF: 1:50-100
- IP: 1:100
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Notes |
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*Predicted MW = 220-240 kDa *use casein or BSA in the blocking solution rather than non-fat-dry milk |
Storage |
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RNA Polymerase II H5 Monoclonal Antibody Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
References |
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Ahn SH, Kim M, Buratowski S. Phosphorylation of serine 2 with the RNA polymerase II c-terminal domain couples transcription and 3’ end processing. Mol. Cell 13:67-76, 2004. Espinosa JM, Verdun RE, Emerson BM. p53 functions through stress- and promoter-specific recruitment of transcription initiation components before and after DNA damage. Mol. Cell. 12(4):1015-27, 2003. Pellizzoni L, Charroux B, Rappsilber J, Mann M, Dreyfuss G. A functional interaction between the survival motor neuron complex and RNA polymerase II. J. Cell. Biol. 152(1):75-85, 2001. Patturajan M, Schulte RJ, Sefton BM, Berezney R, Vincent M, Bensaude O, Warren SL, Corden JL. Growth-related changes in phosphorylation of yeast RNA polymerase II. J. Biol. Chem. 273(8):4689-4694, 1998. Bregman DB, Du L, van der Zee S, Warren SL. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains. J. Cell. Biol. 129:287-298, 1995. |
Warranty/Conditions |
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Covance products may not be resold or modified for resale without prior written approval. |
Rev. Date |
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8/24/2009 RNA Polymerase II H5 Monoclonal Antibody |