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- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
RNA Polymerase II H5 Monoclonal Antibody
- 库存：
1
- 供应商：
上海信裕
- RNA Polymerase II H5 Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal Antibody against RNA Polymerase II Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone H5 Form Ascites Host RNA Polymerase II H5 Monoclonal Antibody Mouse Species Reactivity Extensive (Yeast to Human) IsoType IgM [Ab] 2-3 mg/mL Specificity The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 2 version of pol II. Uses This antibody is effective in immunofluorescence (IF), immunoblotting (WB), immunoprecipitation (IP), pol II purification and functional studies. Suggested Working Dilution Quality control tests were done by Western blot and Dot blot. The optimal working dilution should be determined for each specific assay condition. - WB: 1:500*
- IF: 1:50-100
- IP: 1:100
Notes *Predicted MW = 220-240 kDa
*use casein or BSA in the blocking solution rather than non-fat-dry milkStorage RNA Polymerase II H5 Monoclonal Antibody Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Ahn SH, Kim M, Buratowski S. Phosphorylation of serine 2 with the RNA polymerase II c-terminal domain couples transcription and 3’ end processing. Mol. Cell 13:67-76, 2004.
Espinosa JM, Verdun RE, Emerson BM. p53 functions through stress- and promoter-specific recruitment of transcription initiation components before and after DNA damage. Mol. Cell. 12(4):1015-27, 2003.
Pellizzoni L, Charroux B, Rappsilber J, Mann M, Dreyfuss G. A functional interaction between the survival motor neuron complex and RNA polymerase II. J. Cell. Biol. 152(1):75-85, 2001.
Patturajan M, Schulte RJ, Sefton BM, Berezney R, Vincent M, Bensaude O, Warren SL, Corden JL. Growth-related changes in phosphorylation of yeast RNA polymerase II. J. Biol. Chem. 273(8):4689-4694, 1998.
Bregman DB, Du L, van der Zee S, Warren SL. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains. J. Cell. Biol. 129:287-298, 1995.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. Rev. Date 8/24/2009 RNA Polymerase II H5 Monoclonal Antibody - xy-1453R Caveolin-1细胞质膜微囊蛋白-1抗体
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文献和实验An RNA Polymerase II In Vitro Transcription System
A great deal of attention in recent years has been focused on the mechanisms governing transcription. Repression and/or activation of specific genes, or sets of genes, represents a key regulatory step in such diverse processes as cell growth
Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
polymerase II (Pol II) chromatin immunoprecipitation (ChIP) is a simpler alternative to determine the transcription rate of genes. Moreover, this approach provides more information about the transcriptional regulation of a gene than nuclear run-on. The power
RNA polymerase II (Pol II) plays a crucial role in eukaryotic biology since it is necessary for the expression of all protein-coding genes as well as most microRNAs and several small nuclear RNAs. Pol II is specifically recruited to core
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