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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH conjugated synthetic peptide derived from hu MEK6
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 保存条件:
2-8℃短期保存或<-20℃长期保存1年
- 克隆性:
多克隆
- 适应物种:
hu, mo, rat
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
- 浓度:
1mg/1ml
- 抗体名:
IFN- Gamma(Interferon Gamma)Ag-mouse、rat 干扰素-γ多肽抗原(大、小鼠)
- 规格:
0.2ml/0.1ml/200μg
交叉反应 Hum,Mouse, Rat, Rabbit, pig(更多详情咨询客服)
产品应用 WB=1:100-1000 ELISA=1:1000-5000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100- 500 IF=1:100-500 (石蜡切片需做抗原修复)
保存条件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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文献和实验Thawing Cells - Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. - Add the 1ml
THAWING CELLS Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. Add the 1ml of cell containing media to 9ml of warmed PMEF media
FEEDER PREPARATION BY GAMMA IRRADIATION
FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose, no pyruvate), 7% FBS and 1X GPS. 1. Gelatinize plates
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