四臂聚乙二醇，4-arm PEG-OH

四臂聚乙二醇，4-arm PEG-OH

英文名：4-arm Poly(ethylene glycol)官能化度≥95% PDI<1.05 MW 2000、5000、10000、20000

       多臂官能团产品克服了现有技术中多臂聚乙二醇都只有单一活性基团的现状，提供一种新型的、结构简单且稳定的具有不同类型活性基团的多臂聚乙二醇衍生物。

直链的异双官能团聚乙二醇衍生物在应用上有一定的局限性。用直链的双官能团聚乙二醇衍生物连接的两个分子的比例基本上是1∶1。如果有一个分子需要比另一个分子多，例如，一个分子的体内活性低，这就要求活性低的分子比活性高的分子连接多，这对直链的异双官能团聚乙二醇衍生物就是挑战。同时，在药物承载方面，多臂聚乙二醇相比直链聚乙二醇具有优势。直链的异双官能团聚乙二醇衍生物只能承载两个分子；而多臂聚乙二醇衍生物广泛应用于多肽和小分子药物的聚乙二醇化修饰。

 

物理参数：

英文名称：HO-PEG-FITC，Fluorescein-PEG-OH，Fluorescein-PEG-Hydroxyl

中文名称：羟基聚乙二醇荧光素，异硫氰酸荧光素-聚乙二醇-羟基，荧光素PEG羟基

分子量：1k，2k，3.4k，5k，10k，20k（可按需定制）

性状：固体或液体（根据分子量决定）

规格标准：1g，5g，10g，可提供mg级以及kg级的产品开发服务

储存条件：-20℃，干燥，避免频繁解冻和冷冻

溶解性：溶于大部分有机溶剂，溶于水

 

简述：

Fluorescein-PEG-OH是由三部分组成：荧光素、聚乙二醇和羟基。荧光素是一种能够吸收光能并发出荧光的物质，使得该试剂具有良好的荧光特性。聚乙二醇则是一种长链聚合物，具有良好的生物相容性和水溶性。而羟基则赋予了该试剂一定的反应活性，使其能够与其他分子进行化学反应。

Fluorescein-PEG-OH可以用来标记生物分子，如蛋白质、核酸等。  

Fluorescein-PEG-OH通常是通过荧光素和PEG的化学反应合成的。  

Fluorescein-PEG-OH因其良好的荧光特性和良好的生物相容性而被广泛使用。通过与生物分子（如蛋白质、核酸等）结合，这种试剂可以实现对生物分子的可视化追踪和监测。   

    English name: 4-arm Poly (ethylene glycol) Functionality ≥ 95% PDI<1.05 MW 2000, 5000, 10000, 20000
Multi arm functional group products overcome the current situation where multi arm polyethylene glycol only has a single active group in existing technology, providing a new, structurally simple and stable multi arm polyethylene glycol derivative with different types of active groups.
The application of polyethylene glycol derivatives with straight chain heterobifunctional groups has certain limitations. The ratio of two molecules connected by straight chain bifunctional polyethylene glycol derivatives is basically 1:1. If a molecule needs to have more connections than another molecule, for example, if one molecule has lower in vivo activity, it requires the molecule with lower activity to have more connections than the molecule with higher activity, which poses a challenge for straight chain heterobifunctional polyethylene glycol derivatives. Meanwhile, in terms of drug delivery, multi arm polyethylene glycol has advantages over straight chain polyethylene glycol. The straight chain heterobifunctional polyethylene glycol derivatives can only carry two molecules; Multi arm polyethylene glycol derivatives are widely used for polyethylene glycol modification of peptides and small molecule drugs.
Physical parameters:
      English name: HO-PEG-FITC, Fluoroscein PEG-OH, Fluoroscein PEG-Hydroxyl
Chinese name: Hydroxypolyethylene glycol fluorescein, isothiocyanate fluorescein polyethylene glycol hydroxyl, fluorescein PEG hydroxyl
Molecular weight: 1k, 2k, 3.4k, 5k, 10k, 20k (customizable as needed)
Character: Solid or liquid (determined by molecular weight)
Specification standards: 1g, 5g, 10g, can provide product development services for both mg and kg levels
Storage conditions: -20 ℃, dry, avoid frequent thawing and freezing
Solubility: soluble in most organic solvents, soluble in water
Overview:
Fluorescein PEG-OH is composed of three parts: fluorescein, polyethylene glycol, and hydroxyl group. Fluorescein is a substance that can absorb light energy and emit fluorescence, giving the reagent good fluorescence characteristics. Polyethylene glycol is a long-chain polymer with good biocompatibility and water solubility. The hydroxyl group endows the reagent with certain reactivity, allowing it to undergo chemical reactions with other molecules.
Fluorescein PEG-OH can be used to label biomolecules such as proteins, nucleic acids, etc. 
Fluorescein PEG-OH is usually synthesized through a chemical reaction between fluorescein and PEG. 
Fluorescein PEG-OH is widely used due to its excellent fluorescence properties and good biocompatibility. By combining with biomolecules such as proteins and nucleic acids, this reagent can achieve visual tracking and monitoring of biomolecules. 

 

相关试剂：

Thiol-PEG-FITC 

HO-PEG-Alkyne

HO-PEG-Amine

HO-PEG-SH

 

PEG分子量可选：350、550、750、1k、2k、34k、5k

生物医学应用：PEG-OH 具有良好的生物相容性，常用于药物传递、纳米药物载体、蛋白质和抗体的稳定和保护，以及生物传感器等生物医学应用。

化学修饰剂：PEG-OH 中的羟基官能团可以参与化学反应，例如酯化、醚化、胺基化等，从而实现对PEG的化学修饰，添加其他官能团或功能分子。

PEG-OH 可以通过控制聚合物的分子量和羟基的数量来调节其性质和应用。较长的PEG链段可以提供更好的溶解性和生物稳定性，而羟基的数量则可以影响其反应性和化学修饰的能力。

 

四臂聚乙二醇，4-arm PEG-OH

FITC-PEG-OH的合成通常涉及到PEG链的修饰和FITC的共价连接。下面是一种常见的合成方法和步骤：

1. 材料准备：

   - PEG：选择适当分子量的PEG作为起始材料，常见的分子量范围为2,000 Da至20,000 Da。

   - FITC：购买荧光素异硫氰酸酯（FITC）作为荧光染料。

 

2. PEG修饰：

   - PEG羟基化：通过在PEG末端引入羟基（-OH），可使用PEG-OH或PEG-COOH等。

   - 活化剂处理：将PEG-OH与活化剂（如活化的二）反应，形成活性的PEG活化物。

 

3. FITC连接：

   - FITC反应：将活性的PEG活化物与FITC反应，使FITC与PEG发生共价结合。反应可以在碱性条件下进行，如在碳酸氢钠（NaHCO3）缓冲液中。

   - 反应时间和温度：根据反应条件的选择，调整反应时间和温度。通常，反应时间为数小时至过夜，温度为室温或低温。

 

4. 纯化和分离：

   - 反应停止：使用适当的试剂（如甲醇或甲醛）停止反应，以去除未反应的FITC。

   - 纯化：采用适当的纯化方法（如溶剂沉淀、洗涤、透析或柱层析）来纯化和分离FITC-PEG-OH产物。

 

5. 表征和验证：

   - 使用核磁共振（NMR）等技术验证合成产物的结构和纯度。

   - 可使用荧光光谱仪验证FITC-PEG-OH的荧光性质和荧光强度。

 

需要注意的是，具体的合成方法和步骤可能因实验室条件和研究需求而有所差异。在进行合成时，应遵循适当的实验室安全操作和合成技术，确保产物的质量和稳定性。

The synthesis of FITC-PEG-OH typically involves modification of PEG chains and covalent linkage of FITC. Here is a common synthesis method and step:
1. Material preparation:
-PEG: Choose an appropriate molecular weight of PEG as the starting material, with a common molecular weight range of 2000 Da to 20000 Da.
-FITC: Purchase fluorescein isothiocyanate (FITC) as a fluorescent dye.
2. PEG modification:
-PEG hydroxylation: By introducing hydroxyl groups (- OH) at the end of PEG, PEG-OH or PEG-COOH can be used.
-Activator treatment: PEG-OH reacts with an activator (such as activated disulfide anhydride) to form an active PEG activator.
3. FITC connection:
-FITC reaction: The active PEG activator is reacted with FITC to covalently bind FITC with PEG. The reaction can be carried out under alkaline conditions, such as in sodium bicarbonate (NaHCO3) buffer solution.
-Reaction time and temperature: Adjust the reaction time and temperature according to the selection of reaction conditions. Usually, the reaction time is several hours to overnight, and the temperature is room temperature or low temperature.
4. Purification and Separation:
-Reaction stop: Use appropriate reagents (such as methanol or formaldehyde) to stop the reaction and remove unreacted FITC.
-Purification: Use appropriate purification methods (such as solvent precipitation, washing, dialysis, or column chromatography) to purify and separate the FITC-PEG-OH product.
5. Characterization and validation:
-Verify the structure and purity of the synthesized product using techniques such as nuclear magnetic resonance (NMR).
-The fluorescence properties and intensity of FITC-PEG-OH can be verified using a fluorescence spectrometer.
It should be noted that the specific synthesis methods and steps may vary depending on laboratory conditions and research needs. During synthesis, appropriate laboratory safety procedures and synthesis techniques should be followed to ensure the quality and stability of the product.

 

Cy7.5 NHS ester

cy7-葡聚糖500kd

CY3-标记转铁蛋白

HA50K-CY5

HA10K-CY5

HA500K-CY5.5

HA100K-CY5

HA1200K-CY5

CY5-D-葡萄糖

Cypate-16：0 Lyso：PC

CY7-Dextran MW:2000K

cy3.5 NH2

cy5.5 N3

Sulfo CY3 COOH

CY3-PEG-MAL, MW:2K

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