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- 文献和实验
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- 保存条件:
蛋白表达区间:Val29-Asn315
- 保质期:
See instructions
- 英文名:
标签:C-6His
- 库存:
表达系统:Human Cells
- 供应商:
上海经科化学科技有限公司
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥760.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥2000.0 |
| 规格: | 500ug | 产品价格: | ¥12320.0 |
| 规格: | 1mg | 产品价格: | ¥17600.0 |
- Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
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文献和实验Limbic system-associated membrane protein is also known as LSAMP, IgLON family member 3. In humans, it is encoded by the LSAMP gene. It belongs to the immunoglobulin superfamily and contains 3 Ig-like C2-type domains. Limbic system-associated membrane protein mediates selective neuronal growth and axon targeting. It contributes to the guidance of developing axons and remodeling of mature circuits in the limbic system. It is also essential for normal growth of the hyppocampal mossy fiber projection
LAMP引物设计的在线网站(http://primerexplorer.jp/e/),只要导入靶基因就能自动生成成组引物。 以某一微生物的鞭毛基因为例讲解一下LAMP相物设计的过程: 首先单击浏览钮择靶基因序列文件,靶序列默认的是小于2 2 kbp。支持三个类型的文件,普通文本格式(仅含序列), FASTA格式和GenBank 格式文件。 第二,从下面个项中择定参数设定(引物设计条件条件。基于GC含量的自动判断, 起始的参数是特定的:如果GC含量小于或等于45%.,则选取AT丰度高的区
1.LAMP引物的设计LAMP引物的设计主要是针对靶基因的六个不同的区域,基于靶基因3' 端的F3c、F2c和Flc区以及5' 端的Bl、B2和B3区等6个不同的位点设计4种引物。FIP(Forward Inner Primer):上游内部引物,由F2区和F1C区域组成,F2区与靶基因3’端的F2c区域互补,F1C区与靶基因5' 端的Flc区域序列相同。F3引物:上游外部引物(Forward Outer Primer),由F3区组成,并与靶基因的F3c区域互补。BIP引物:下游内部引物
PCR方法在人类及动植物疾病基因诊断、食品分析和环境监测等领域发挥着举足轻重的作用,其灵敏度高、特异性好,是目前最精准的基因诊断方法。然而PCR方法操作起来较复杂,对仪器和人员要求比较高,不适合基层或现场快速诊断,因此在国内的推广速度并不是很快。 2000年日本学者Notomi在Nucleic Acids Res杂志上公开了一种新的基因诊断技术,即LAMP (Loop-mediated isothermal amplification),中文名为“环介导等温扩增反应”,受到了世卫
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