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- 2025年07月14日
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- 文献和实验
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- 保存条件：
4℃保存
- 保质期：
6个月
- 库存：
1
- 供应商：
上海信裕
- DNA浓缩试剂盒详细信息：
-
Product Overview
The DNA Concentrator Kit is a complete set of essential components which enables the experimenter to efficiently concentrate DNA from various DNA samples with low concentration of DNA including those from microdissection samples and paraffin-embedded tissues. The entire procedure can be completed within 2 minutes and produces far superior results than any competitor kits. The DNA Concentrator Kit is suitable for concentrating DNA from various DNA samples, especially from those with low concentration of DNA such as microdissection samples, paraffin-embedded tissues, restriction enzyme digestions, PCR reactions, and home-made minipreps.
WHY CHOOSE THE DNA CONCENTRATOR KIT?
- The fastest procedure available (< 2 minutes).
- Concentrated DNA is ultra-pure with efficient removal of contaminants (salts, proteins, enzymes, nucleotides and primers).
- Use of user-friendly binding solution and no chaotropic or other irritant reagents.
DNA浓缩试剂盒Principle & Procedure
The DNA Concentrator Kit simply apply our proprietary DNA binding buffer to DNA sample. After transferring the sample to the specially designed Fast-Spin Column and washing with washing buffer, the DNA is easily recovered in 8-10 µl with elution solution. Concentrated DNA is ready for down-stream application.
SCHEMATIC PROCEDURE: 
DNA浓缩试剂盒Product Components
CA1 (binding buffer)
CA2 (wash buffer)
CA3 (DNA elution buffer)
F-spin Column
F-collection tube
User guide
90508-20 包涵体微量纯化试剂盒
90509-20 包涵体中量纯化试剂盒
90510-2 包涵体大量纯化试剂盒
100302-100 包涵体蛋白溶解及复性套装
90613-25 细胞核蛋白微量制备试剂盒
90613-100 细胞核蛋白微量制备试剂盒
91203-30 一步式胞浆蛋白微量制备试剂盒
91203-100 一步式胞浆蛋白微量制备试剂盒
100202-100 HRP标记的抗His标签抗体
100101-2 His标记蛋白质专用镍柱介质
100101-10 His标记蛋白质专用镍柱介质
100102-20 一站式His标记蛋白质微量纯化套装
90902-30 细菌蛋白质微量提取试剂盒
81202-50 酵母蛋白质快速微量提取试剂盒
80814-100 Bradford法蛋白定量试剂盒
80814-200 Bradford法蛋白定量试剂盒
90310-1 BSA蛋白浓度标准
80815-500 BCA法蛋白定量试剂盒
80816-500 超敏型BCA法蛋白定量试剂盒
81029-10 非变性法蛋白浓缩试剂盒
81217 TCA微量蛋白沉淀浓缩试剂
100913 TCA-DOC微量蛋白沉淀浓缩试剂
100914 TCA-丙酮微量蛋白沉淀浓缩试剂
100915 丙酮微量蛋白沉淀浓缩试剂
81214-10 Tricine-SDS-PAGE电泳液（干粉）
81205-30 一站式Tricine-SDS-PAGE套装
80806-100 无浓缩胶PAGE制胶液
81221-30 无浓缩胶SDS-PAGE电泳套装
80810-30 一站式SDS-PAGE蛋白电泳套装
100867-500 SDS-PAGE浓缩胶配胶液
100868-500 SDS-PAGE分离胶配胶液
81204-1 SDS-PAGE上样液
81204-10 SDS-PAGE上样液
81203-5 SDS-PAGE电泳液干粉DNA浓缩试剂盒User Guide & MSDS
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文献和实验Amicon Ultra-0.5 mL Centrifugal Filters for DNA Purification and Concentration 操作示意图： With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times
刚才收集到的上层液，重复步骤4。 6) 加入200 ml chloroform萃取上层液，并重复步骤4。 浓缩质体DNA 1.仪器用具： a.桌上型离心机 b.真空干燥器 2.药品及试剂： a.3 M sodium acetate (NaOAc), pH 5.2 b.100% ethanol c.TE buffer 3.方法步骤: 1)加入1/10体积的sodium acetate于DNA溶液中，使其最终浓度为0.3 M
目的 将复杂的细胞分子混合物加入有机溶媒，除去蛋白质及其它成分，就可以纯化DNA。一般常用酚及氯仿(phenol/chloroform)可使蛋白质变性(denaturaion)的特性来进行萃取的步骤，DNA和RNA不溶于有机溶媒中，而溶于水层。另外，分子选殖(molecular cloning)常利用洋菜胶(agarose)来分离不同大小的DNA片段或用以纯化DNA。 DNA经限制酶反应、自胶体中溶离或任何酵素反应后，常常体积遽增，为了浓缩已经纯化的DNA，常利用酒精沉淀法来制备，经过离心
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