染色质免疫沉淀(ChIP)试剂盒

The EpiQuik™ Chromatin Immunoprecipitation (ChIP) Kit is a convenient package of tools that allows the experimenter to perform chromatin immunoprecipitation (ChIP) at extraordinarily rapid speeds and consistency,superior to all other current ChIP methods available. The kit is ready-to-use and provides all the essential components needed to carry out a successful ChIP experiment. The EpiQuik™ ChIP kits are suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ CHROMATIN IMMUNOPRECIPITATION CHIP KIT?

• Straightforwardly put: It is the truly number one fastest procedure available. The entire procedure can be completed within an amazingly short 5 hour period with superb results.
• The ChIP procedure has been drastically simplified -- extremely short and easy to follow.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Columns for DNA purification are included: save tremendous amounts of time and reduce unnecessary physical labor.
• Compatible with all DNA amplification-based approaches.
• Achieves very reliable and consistent assay conditions.

This kit includes a positive control antibody (RNA polymerase II), a negative control normal mouse IgG, and GAPDH primers that can be used as a positive control to demonstrate the efficacy of the kit reagents and protocol. RNA polymerase II is considered to be enriched in the GAPDH gene promoter that is expected to be undergoing transcription in most growing mammalian cells and can be immunoprecipitated by RNA polymerase II but not by normal mouse IgG.

In this ChIP, cells are cross-linked with formaldehyde and chromatin is extracted. The chromatin is then sheared and added into the microwell immobilized with affinity antibodies. Cross-linked DNA is released from antibody-captured protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

SCHEMATIC PROCEDURE:	COMPARATIVE OVERVIEW:

CP1 (wash buffer)
CP2 (antibody buffer)
CP3A (lysis buffer)
CP3B (lysis buffer)
CP4 (ChIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Protease inhibitor cocktail (100x)*
Normal mouse IgG (1 mg/ml)*
Anti-RNA polymerase II (1mg/ml)*
Proteinase K (10 mg/ml)*
Control primer (GAPDH)
Forward (20 µM)*
Reverse (20 µM)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide
xy315-2 羊抗兔IgG（全分子）-FITC
xy315-3 羊抗兔IgG（全分子）-FITC
xy316-1 羊抗兔（全分子）-RBITC
xy316-2 羊抗兔（全分子）-RBITC
xy316-3 羊抗兔（全分子）-RBITC
xy317-1 羊抗小鼠IgG（全分子）-FITC
xy317-2 羊抗小鼠IgG（全分子）-FITC
xy317-3 羊抗小鼠IgG（全分子）-FITC
xy319-1 羊抗小鼠IgG-RBITC
xy319-2 羊抗小鼠IgG-RBITC
xy319-3 羊抗小鼠IgG-RBITC
xy320-1 羊抗大鼠IgG-FITC
xy320-2 羊抗大鼠IgG-FITC
xy320-3 羊抗大鼠IgG-FITC
xy321-1 羊抗大鼠IgG-RBITC
xy321-2 羊抗大鼠IgG-RBITC
xy321-3 羊抗大鼠IgG-RBITC
xy322-1 羊抗猪IgG-FITC
xy322-2 羊抗猪IgG-FITC
xy323-1 羊抗猪IgG-RBITC
xy323-2 羊抗猪IgG-RBITC
xy324-1 羊抗牛IgG-FITC
xy325-1 羊抗牛IgG-RBTIC
xy326-1 羊抗马IgG-FITC
xy327-1 羊抗马IgG-RBITC
xy328-1 羊抗鸡IgG-FITC
xy329-1 羊抗鸡IgG-RBITC
xy330-1 兔抗猴IgG-FITC
xy331-1 兔抗猴IgG-RBITC
xy332-1 兔抗羊IgG-FITC
xy333-1 兔抗羊IgG-RBITC
xy334-1 兔抗狗IgG-FITC
xy335-1 兔抗豚鼠IgG-RBITC
xy336-1 兔抗豚鼠IgG-FITC
xy337-1 BSA-FTIC
* Spin the solution down to the bottom before use.