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- 上海钰博
- 进口/国产
- YB027Hu02
- 2025年07月15日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Erythropoietin Receptor (EPOR)
- 库存：
10000
- 供应商：
钰博生物
红细胞生成素受体(EPOR)重组蛋白
适用生物 Homo sapiens (Human，人)
红细胞生成素受体(EPOR)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 51.5kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybH280Hu01 垂体肿瘤转化1相互作用蛋白(PTTG1IP)重组蛋白 Recombinant Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) Homo sapiens (Human)
ybH314Hu01 cGMP依赖性蛋白激酶Ⅰ(PRKG1)重组蛋白 Recombinant Protein Kinase, cGMP Dependent Type I (PRKG1) Homo sapiens (Human)
ybH319Hu01 脯氨酸/精氨酸丰富端亮氨酸丰富重复蛋白(PRELP)重组蛋白 Recombinant Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP) Homo sapiens (Human)
ybH334Mu01 旁血小板溶蛋白(PPL)重组蛋白 Recombinant Periplakin (PPL) Mus musculus (Mouse)
ybH337Ra01 过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)重组蛋白 Recombinant Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) Rattus norvegicus (Rat)
ybH339Hu01 骨膜蛋白(POSTN)重组蛋白 Recombinant Periostin (POSTN) Homo sapiens (Human)
ybH358Hu01 丛状蛋白B1(PLXNB1)重组蛋白 Recombinant Plexin B1 (PLXNB1) Homo sapiens (Human)
ybH363Hu01 质膜膜泡关联蛋白(PLVAP)重组蛋白 Recombinant Plasmalemma Vesicle Associated Protein (PLVAP) Homo sapiens (Human)
ybH364Mu01 腭/肺/鼻上皮癌关联蛋白(PLUNC)重组蛋白 Recombinant Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) Mus musculus (Mouse)
ybH366Hu01 丝束蛋白1(PLS1)重组蛋白 Recombinant Plastin 1 (PLS1) Homo sapiens (Human)
ybH380Hu01 多型性腺瘤基因样蛋白1(PLAGL1)重组蛋白 Recombinant Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1) Homo sapiens (Human)
ybH413Hu01 磷酸组氨酸性磷酸酶1(PHPT1)重组蛋白 Recombinant Phosphohistidine Phosphatase 1 (PHPT1) Homo sapiens (Human)
适用生物 Homo sapiens (Human，人)
红细胞生成素受体(EPOR)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 51.5kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Erythropoietin Receptor (EPOR)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPA027Hu02 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 51.5kDa |
| Predicted isoelectric point | 5.8 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | Cell membrane; Single-pass type I membrane protein. |
| Residues | Pro33~Leu210 (Accession # P19235) with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-PKFESKAA LLAARGPEEL LCFTERLEDL VCFWEEAASA GVGPGNYSFS YQLEDEPWKL CRLHQAPTAR GAVRFWCSLP TADTSSFVPL ELRVTAASGA PRYHRVIHIN EVVLLDAPVG LVARLADESG HVVLRWLPPP ETPMTSHIRY EVDVSAGNGA GSVQRVEILE GRTECVLSNL
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-PKFESKAA LLAARGPEEL LCFTERLEDL VCFWEEAASA GVGPGNYSFS YQLEDEPWKL CRLHQAPTAR GAVRFWCSLP TADTSSFVPL ELRVTAASGA PRYHRVIHIN EVVLLDAPVG LVARLADESG HVVLRWLPPP ETPMTSHIRY EVDVSAGNGA GSVQRVEILE GRTECVLSNL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
红细胞生成素受体(EPOR)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
红细胞生成素受体(EPOR)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybH274Hu01 谷氨酰胺酰肽环转移酶(QPCT)重组蛋白 Recombinant Glutaminyl Peptide Cyclotransferase (QPCT) Homo sapiens (Human)
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
红细胞生成素受体(EPOR)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybH280Hu01 垂体肿瘤转化1相互作用蛋白(PTTG1IP)重组蛋白 Recombinant Pituitary Tumor Transforming 1 Interacting Protein (PTTG1IP) Homo sapiens (Human)
ybH314Hu01 cGMP依赖性蛋白激酶Ⅰ(PRKG1)重组蛋白 Recombinant Protein Kinase, cGMP Dependent Type I (PRKG1) Homo sapiens (Human)
ybH319Hu01 脯氨酸/精氨酸丰富端亮氨酸丰富重复蛋白(PRELP)重组蛋白 Recombinant Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP) Homo sapiens (Human)
ybH334Mu01 旁血小板溶蛋白(PPL)重组蛋白 Recombinant Periplakin (PPL) Mus musculus (Mouse)
ybH337Ra01 过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)重组蛋白 Recombinant Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) Rattus norvegicus (Rat)
ybH339Hu01 骨膜蛋白(POSTN)重组蛋白 Recombinant Periostin (POSTN) Homo sapiens (Human)
ybH358Hu01 丛状蛋白B1(PLXNB1)重组蛋白 Recombinant Plexin B1 (PLXNB1) Homo sapiens (Human)
ybH363Hu01 质膜膜泡关联蛋白(PLVAP)重组蛋白 Recombinant Plasmalemma Vesicle Associated Protein (PLVAP) Homo sapiens (Human)
ybH364Mu01 腭/肺/鼻上皮癌关联蛋白(PLUNC)重组蛋白 Recombinant Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) Mus musculus (Mouse)
ybH366Hu01 丝束蛋白1(PLS1)重组蛋白 Recombinant Plastin 1 (PLS1) Homo sapiens (Human)
ybH380Hu01 多型性腺瘤基因样蛋白1(PLAGL1)重组蛋白 Recombinant Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1) Homo sapiens (Human)
ybH413Hu01 磷酸组氨酸性磷酸酶1(PHPT1)重组蛋白 Recombinant Phosphohistidine Phosphatase 1 (PHPT1) Homo sapiens (Human)
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文献和实验相关实验
酸激酶（protein tyrosine kinase,PTK）结构。 （2）IL-1Rt I和IL-1RtⅡ：胞膜外区均含有3个C2样结构，受体胞浆区丝氨酸/苏氨酸磷酸化可能与受体介导的信号转导有关。 （3）IL-6Rα链、gp130以及G-CSFR：胞膜外区N端 均含1个C2样区，在靠近胞膜侧各有1个红细胞生成素受体超家族结构域，此外在胞有胞膜外区还含有2～4个纤粘连素结构域。gp130胞浆区酷氨酸磷酸化与信号转导有关。这种结构类型的受体其相应配体IL-6、OSM、LIF和G-CSF在氨基酸序列
-6、G-CSF、EPO、IL-7的信号转导起着关键作用，提示这些受体可能利用相似的胞膜内信号转导机制。 1.gp130介导信号转导 在IL-6R、IL-11R、OSMR、LIFR、CNTFR的信号转导共用链gp130中，其胞浆区约277个氨基酸残基中包含丝氨酸富含区、核苷酸结合区及4个GTP结合模式区。其中的丝氨酸富含区也存在于G-CSFR、IL-2Rβ、IL-4R和EPOR，其它的ERS成员有着明显的同源性。其中一个片段在所有ERS成员中都是保守的，另一个片段存在于G-CSFR
吴佰霖 老板的一个课题，一个分泌性蛋白，可结合一些小分子，与膜上受体结合，但是用重组表达的蛋白与 pcDNA过表达这个蛋白的生物学效果不一样 请问有什么研究策略吗 freecell 你的研究材料？ 研究目的？ 吴佰霖 材料：细胞系，酵母表达重组蛋白，pcDNA表达克隆载体， 研究目的是分泌性蛋白结合受体后在胞内的信号通路，或相关作用分子
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红细胞生成素受体(EPOR)重组蛋白
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