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- YB348Mu01
- 2025年07月15日
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- 详细信息
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- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Cathepsin A (CTSA)
- 库存：
10000
- 供应商：
钰博生物
组织蛋白酶A(CTSA)重组蛋白
适用生物 Mus musculus (Mouse，小鼠)
组织蛋白酶A(CTSA)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 61.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybF237Hu01 L-型电压依赖性钙离子通道α1D亚基(CACNα1D)重组蛋白 Recombinant Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) Homo sapiens (Human)
ybF255Hu01 钙蛋白酶6(CAPN6)重组蛋白 Recombinant Calpain 6 (CAPN6) Homo sapiens (Human)
ybF264Hu01 羰基还原酶3(CBR3)重组蛋白 Recombinant Carbonyl Reductase 3 (CBR3) Homo sapiens (Human)
ybF292Mu01 封闭蛋白2(CLDN2)重组蛋白 Recombinant Claudin 2 (CLDN2) Mus musculus (Mouse)
ybF293Hu01 封闭蛋白3(CLDN3)重组蛋白 Recombinant Claudin 3 (CLDN3) Homo sapiens (Human)
ybF294Ra01 封闭蛋白4(CLDN4)重组蛋白 Recombinant Claudin 4 (CLDN4) Rattus norvegicus (Rat)
ybF295Ra01 封闭蛋白5(CLDN5)重组蛋白 Recombinant Claudin 5 (CLDN5) Rattus norvegicus (Rat)
ybF299Hu01 封闭蛋白11(CLDN11)重组蛋白 Recombinant Claudin 11 (CLDN11) Homo sapiens (Human)
ybF303Hu01 细胞因子受体样因子1(CRLF1)重组蛋白 Recombinant Cytokine Receptor Like Factor 1 (CRLF1) Homo sapiens (Human)
ybF315Ra01 羧肽酶A1(CPA1)重组蛋白 Recombinant Carboybpeptidase A1, Pancreatic (CPA1) Rattus norvegicus (Rat)
ybF316Ra01 羧肽酶A2(CPA2)重组蛋白 Recombinant Carboybpeptidase A2, Pancreatic (CPA2) Rattus norvegicus (Rat)
ybF317Hu01 羧肽酶A4(CPA4)重组蛋白 Recombinant Carboybpeptidase A4 (CPA4) Homo sapiens (Human)
ybF320Ra01 羧肽酶B1(CPB1)重组蛋白 Recombinant Carboybpeptidase B1, Tissue (CPB1) Rattus norvegicus (Rat)
ybF322Hu01 羧肽酶E(CPE)重组蛋白 Recombinant Carboybpeptidase E (CPE) Homo sapiens (Human)
ybF323Hu01 羧肽酶N1(CPN1)重组蛋白 Recombinant Carboybpeptidase N1 (CPN1) Homo sapiens (Human)
适用生物 Mus musculus (Mouse，小鼠)
组织蛋白酶A(CTSA)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 61.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Cathepsin A (CTSA)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | RPA348Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 61.4kDa |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Asp215~Met470 (Accession # P10619) with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-DNSLVY FAYYHGLLGN RLWSSLQTHC CSQNKCNFYD NKDLECVTNL QEVARIVGNS GLNIYNLYAP CAGGVPSHFR YEKDTVVVQD LGNIFTRLPL KRMWHQALLR SGDKVRMDPP CTNTTAASTY LNNPYVRKAL NIPEQLPQWDMCNFLVNLQY RRLYRSMNSQ YLKLLSSQKY QILLYNGDVD MACNFMGDEW FVDSLNQKMEVQRRPWLVKY GDSGEQIAGF VKEFSHIAFL TIKGAGHMVP TDKPLAAFTM
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-DNSLVY FAYYHGLLGN RLWSSLQTHC CSQNKCNFYD NKDLECVTNL QEVARIVGNS GLNIYNLYAP CAGGVPSHFR YEKDTVVVQD LGNIFTRLPL KRMWHQALLR SGDKVRMDPP CTNTTAASTY LNNPYVRKAL NIPEQLPQWDMCNFLVNLQY RRLYRSMNSQ YLKLLSSQKY QILLYNGDVD MACNFMGDEW FVDSLNQKMEVQRRPWLVKY GDSGEQIAGF VKEFSHIAFL TIKGAGHMVP TDKPLAAFTM
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
组织蛋白酶A(CTSA)重组蛋白 Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
组织蛋白酶A(CTSA)重组蛋白 Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
组织蛋白酶A(CTSA)重组蛋白 Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybF219Hu01 外核苷酸焦磷酸酶/磷酸二酯酶1(ENPP1)重组蛋白 Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) Homo sapiens (Human)
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybF237Hu01 L-型电压依赖性钙离子通道α1D亚基(CACNα1D)重组蛋白 Recombinant Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) Homo sapiens (Human)
ybF255Hu01 钙蛋白酶6(CAPN6)重组蛋白 Recombinant Calpain 6 (CAPN6) Homo sapiens (Human)
ybF264Hu01 羰基还原酶3(CBR3)重组蛋白 Recombinant Carbonyl Reductase 3 (CBR3) Homo sapiens (Human)
ybF292Mu01 封闭蛋白2(CLDN2)重组蛋白 Recombinant Claudin 2 (CLDN2) Mus musculus (Mouse)
ybF293Hu01 封闭蛋白3(CLDN3)重组蛋白 Recombinant Claudin 3 (CLDN3) Homo sapiens (Human)
ybF294Ra01 封闭蛋白4(CLDN4)重组蛋白 Recombinant Claudin 4 (CLDN4) Rattus norvegicus (Rat)
ybF295Ra01 封闭蛋白5(CLDN5)重组蛋白 Recombinant Claudin 5 (CLDN5) Rattus norvegicus (Rat)
ybF299Hu01 封闭蛋白11(CLDN11)重组蛋白 Recombinant Claudin 11 (CLDN11) Homo sapiens (Human)
ybF303Hu01 细胞因子受体样因子1(CRLF1)重组蛋白 Recombinant Cytokine Receptor Like Factor 1 (CRLF1) Homo sapiens (Human)
ybF315Ra01 羧肽酶A1(CPA1)重组蛋白 Recombinant Carboybpeptidase A1, Pancreatic (CPA1) Rattus norvegicus (Rat)
ybF316Ra01 羧肽酶A2(CPA2)重组蛋白 Recombinant Carboybpeptidase A2, Pancreatic (CPA2) Rattus norvegicus (Rat)
ybF317Hu01 羧肽酶A4(CPA4)重组蛋白 Recombinant Carboybpeptidase A4 (CPA4) Homo sapiens (Human)
ybF320Ra01 羧肽酶B1(CPB1)重组蛋白 Recombinant Carboybpeptidase B1, Tissue (CPB1) Rattus norvegicus (Rat)
ybF322Hu01 羧肽酶E(CPE)重组蛋白 Recombinant Carboybpeptidase E (CPE) Homo sapiens (Human)
ybF323Hu01 羧肽酶N1(CPN1)重组蛋白 Recombinant Carboybpeptidase N1 (CPN1) Homo sapiens (Human)
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文献和实验相关实验
重组蛋白A在免疫试剂应用中的新进展广州精达公司-美国免疫学博士 胡勇摘要 抗体是免疫诊断试剂领域最重要的原料，蛋白A是纯化抗体最有效的工具，广泛用于免疫诊断试剂的许多领域。重组蛋白A具有产量大，成本低，纯度高，特异性好的优点。重组蛋白A的国产化将极大促进免疫诊断试剂的产品开发和市场效益。 关键词 抗体纯化 免疫诊断试剂 重组蛋白A 国产化蛋白A（Protein A）为金黄色葡萄球菌细胞壁蛋白，具有与人类和哺乳动物血液中免疫球蛋白IgG分子的Fc段特异性结合
问：请问各位高手，我想让酵母蛋白酶Ａ失活，决定采用ＰＣＲ进行ＤＮＡ序列的突变，此法行的通吗？可有前人研究过？ 答：国外有了"蛋白酶缺失"的用于重组蛋白表达的菌株。国内也许也有，小龙对于上游不大懂，不知道自己构建蛋白酶缺失的酵母菌是否有技术上的难度。参见一篇综述《生命的化学》2003 年23 卷1 期CHEMISTRY OF L IFE 2003，23(1) 酵母表达系统及其应用研究。 假如只是为了降低重组蛋白表达时被水解的可能，在提取液中加入蛋白酶抑制剂如PMSF可以部分解决。
Nature 子刊：中国团队在扇贝足丝蛋白仿生材料研究领域取得重要研究进展
是材料领域的研究热点和难点。为弥补当前组织修复材料、柔性传感器和可穿戴设备材料在湿环境下延伸性差、恢复性差等不足，研究团队多年来聚焦在湿环境下具有高延展性的扇贝足丝，克服了天然材料提取表征困难等技术难题，从扇贝足丝蛋白中首次报道了一种具有高延展性的纤维蛋白材料 Sbp5-2，并联合开展了材料组装机制及应用研究，该研究加深了对蛋白基海洋生物材料组装分子机制的认识，为未来开发具有自主知识产权的新型海洋生物医用生物材料奠定了基础。 首先对扇贝足丝结构和机械性能进行表征。研究发现其在湿环境下延伸性能可达
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组织蛋白酶A(CTSA)重组蛋白
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