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发货周期:现货
博士德生物
cell culture supernates and cell lysates.
Mouse
96T
货号 | EK0849 |
---|---|
产品名称 | Mouse TrkB ELISA Kit |
产品描述 | For quantitative detection of mouse TrkB in cell culture supernates and cell lysates. |
免疫原 | NSO,C32-H429 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 93.8pg/ml-6000pg/ml |
基因名称 | NTRK2 |
基因全名 | neurotrophic tyrosine kinase, receptor, type 2 |
Uniprot ID | P15209 |
指标别名 | GP145 TrkB | GP145-TrkB | NTRK2 | Q16620 | Trk-B | TrkB tyrosine kinase |
背景资料 | TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3. The standard product used in this kit is recombinant TrkB, a 49.5KDa glycoprotein, C32—H439. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0849-CAP | 96-well plate precoated with anti- mouse TrkBantibody | 1 |
EK0849-ST | Lyophilized recombinant mouse TrkB standard | 10ng/tube×2 |
EK0849-DA | Biotinylated anti- mouse TrkB antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 188 | 679 | 2008 | 202 | 622 | 2201 |
Standard deviation | 12.22 | 46.85 | 128.51 | 13.73 | 46.02 | 149.66 |
CV(%) | 6.5% | 6.9% | 6.4% | 6.8% | 7.4% | 6.8% |
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.045 | 0.159 | 0.263 | 0.358 | 0.682 | 1.242 | 1.694 | 2.329 |
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