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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Rat
96T
货号 | EK1302 |
---|---|
产品名称 | Rat TIMP-2 ELISA Kit |
产品描述 | For quantitative detection of rat TIMP-2 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, C27-P220 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | TIMP2 |
基因全名 | TIMP metallopeptidase inhibitor 2 |
Uniprot ID | P30121 |
指标别名 | CSC 21K|CSC21K|D11Bwg1104e|DDC8|Metalloproteinase inhibitor 2|Metalloproteinase inhibitor 2 precursor|MGC105282|OTTMUSP00000004170|OTTMUSP00000004171|RP23-394O9.2|TIMP 2|TIMP metallopeptidase inhibitor 2|Tissue inhibitor of metalloproteinase 2|Tissue inhibitor of metalloproteinases 2 |
背景资料 | TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1302-CAP | 96-well plate precoated with anti- rat TIMP-2 antibody | 1 |
EK1302-ST | Lyophilized recombinant rat TIMP-2 standard | 10ng/tube×2 |
EK1302-DA | Biotinylated anti- rat TIMP-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 240 | 1342 | 4533 | 227 | 1463 | 4769 |
Standard deviation | 10.56 | 68.44 | 339.97 | 10.21 | 95.09 | 443.51 |
CV(%) | 4.4% | 5.1% | 7.5% | 4.5% | 6.5% | 9.3% |
Concentration(pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.080 | 0.123 | 0.177 | 0.233 | 0.386 | 0.917 | 1.401 | 1.938 |
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