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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK1355 |
---|---|
产品名称 | Mouse TFPI ELISA Kit |
产品描述 | For quantitative detection of mouse TFPI in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, L29-N306 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | TFPI |
基因全名 | tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) |
Uniprot ID | O54819 |
指标别名 | EPI|Extrinsic pathway inhibitor|LACI|Lipoprotein-associated coagulation inhibitor|TFPI|TFPI1_HUMAN|Tissue factor pathway inhibitor |
背景资料 | TFPI(Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1355-CAP | 96-well plate precoated with anti- mouse TFPI antibody | 1 |
EK1355-ST | Lyophilized recombinant mouse TFPI standard | 10ng/tube×2 |
EK1355-DA | Biotinylated anti- mouse TFPI antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 310 | 1506 | 4359 | 330 | 1630 | 4238 |
Standard deviation | 20.15 | 99.39 | 217.95 | 22.44 | 123.88 | 216.13 |
CV(%) | 6.5% | 6.6% | 5% | 6.8% | 7.6% | 5.1% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.019 | 0.068 | 0.104 | 0.219 | 0.380 | 0.769 | 1.299 | 1.957 |
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