手机验证
发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma(heparin, EDTA, citrate ).
96T
货号 | EK0859 |
---|---|
产品名称 | Human PAI-1 ELISA Kit |
产品描述 | For quantitative detection of human PAI-1 in cell culture supernates, cell lysates, serum and plasma(heparin, EDTA, citrate ). |
免疫原 | NSO, S22-P402 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | SERPINE1 |
基因全名 | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
Uniprot ID | P05121 |
指标别名 | Endothelial plasminogen activator inhibitor|PAI|PAI-1|PAI1|PAI1_HUMAN|PLANH1|Plasminogen activator inhibitor 1|Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1|Serpin E1|Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1|SERPINE1 |
背景资料 | Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator(tPA) and urokinase(uPA), the activators of plasminogen and hence fibrinolysis(the physiological breakdown of blood clots). It is a serine protease inhibitor(serpin) protein(SERPINE1). PAI-1 is mainly produced by the endothelium(cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome(7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis(a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states(such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0859-CAP | 96-well plate precoated with anti- human PAI-1 antibody | 1 |
EK0859-ST | Lyophilized recombinant human PAI-1 standard | 10ng/tube×2 |
EK0859-DA | Biotinylated anti- human PAI-1antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 203 | 1208 | 5496 | 198 | 1244 | 5980 |
Standard deviation | 12.38 | 71.27 | 423.19 | 15.04 | 95.78 | 520.26 |
CV(%) | 6.1% | 5.9% | 7.7% | 7.6% | 7.7% | 8.7% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.023 | 0.111 | 0.205 | 0.380 | 0.725 | 1.152 | 1.594 | 2.061 |
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