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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0814 |
---|---|
产品名称 | Human MSP/MST1 ELISA Kit |
产品描述 | For quantitative detection of human MSP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Q19-G711 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | MST1 |
基因全名 | macrophage stimulating 1 (hepatocyte growth factor-like) |
Uniprot ID | P26927 |
指标别名 | D3F15S2|D3F15S2h|D9H3F15S2|DNF15S2|DNF15S2h|Hepatocyte growth factor like protein|Hepatocyte growth factor like protein homolog|HGFL|Macrophage stimulating 1|Macrophage stimulatory protein|MSP|MST1|NF15S2 |
背景资料 | Macrophage Stimulating 1(SMT1), also known as Hepatocyte growth factor-like protein(HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3(3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0814-CAP | 96-well plate precoated with anti- human MSP antibody | 1 |
EK0814-ST | Lyophilized recombinant human MSP standard | 10ng/tube×2 |
EK0814-DA | Biotinylated anti- human MSP antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 250 | 1105 | 6318 | 245 | 1017 | 6055 |
Standard deviation | 19.25 | 44.2 | 398.03 | 23.52 | 53.9 | 399.63 |
CV(%) | 7.7% | 4% | 6.3% | 9.6% | 5.3% | 6.6% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.001 | 0.090 | 0.183 | 0.357 | 0.684 | 1.136 | 1.780 | 2.379 |
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