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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).
Mouse
96T
货号 | EK0943 |
---|---|
产品名称 | Mouse MPO ELISA Kit |
产品描述 | For quantitative detection of mouse MPO in cell culture supernates, cell lysates, serum and plasma(heparin, EDTA). |
免疫原 | NSO, M16-T718 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | MPO |
基因全名 | myeloperoxidase |
Uniprot ID | P11247 |
指标别名 | 84 kDa myeloperoxidase|89 kDa myeloperoxidase|MPO|MPO Myeloperoxidase|Myeloperoxidase|Myeloperoxidase heavy chain|Myeloperoxidase light chain|PERM_HUMAN |
背景资料 | Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0943-CAP | 96-well plate precoated with anti- mouse MPO antibody | 1 |
EK0943-ST | Lyophilized recombinant mouse MPO standard | 10ng/tube×2 |
EK0943-DA | Biotinylated anti- mouse MPO antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 354 | 1809 | 5328 | 362 | 1718 | 5171 |
Standard deviation | 19.11 | 115.77 | 266.4 | 24.97 | 137.44 | 346.45 |
CV(%) | 5.4% | 6.4% | 5% | 6.9% | 8% | 6.7% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.034 | 0.070 | 0.108 | 0.184 | 0.427 | 0.660 | 1.378 | 2.095 |
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