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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).
96T
货号 | EK0903 |
---|---|
产品名称 | Human KLK1 ELISA Kit |
产品描述 | For quantitative detection of human KLK1 in cell culture supernates, cell lysates, serum and plasma(heparin, EDTA). |
免疫原 | NSO, I25-S262 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | KLK1 |
基因全名 | kallikrein 1 |
Uniprot ID | P06870 |
指标别名 | Glandular kallikrein 1|hK1|Kallikrein 1 renal/pancreas/salivary|Kallikrein serine protease 1|Kallikrein-1|Kidney/pancreas/salivary gland kallikrein|KLK1|KLK1_HUMAN|Klk6|KLKR|Tissue kallikrein |
背景资料 | Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0903-CAP | 96-well plate precoated with anti- human KLK1 antibody | 1 |
EK0903-ST | Lyophilized recombinant human KLK1 standard | 10ng/tube×2 |
EK0903-DA | Biotinylated anti- human KLK1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 304 | 1654 | 3697 | 280 | 1605 | 3442 |
Standard deviation | 12.46 | 71.12 | 284.66 | 16.24 | 93.09 | 271.91 |
CV(%) | 4.1% | 4.3% | 7.7% | 5.8% | 5.8% | 7.9% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.016 | 0.084 | 0.157 | 0.322 | 0.513 | 0.965 | 1.516 | 2.020 |
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