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- 技术资料
- 库存:
大量
- 供应商:
北京智杰方远
- 规格:
500ml

适用于哺乳动物及昆虫细胞的培养,针对杂交瘤细胞以及肿瘤细胞均能普遍适用!
Application
FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
General description
FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.
Linkage
Tired of managing your serum needs? We can assist with your serum planning. Please visit the Serum Planner for more information.
性质
| Related Categories | Apoptosis and Cell Cycle, Core Bioreagents, Fetal Bovine Serum (FBS), General Reagents, Hybridoma Platform, |
| Quality Level | GMP |
| sterility | sterile-filtered |
| composition | Hemoglobin, ≤20 mg/dL |
| origin | USA origin |
| impurities | ≤10 EU/mL endotoxin |
| suitability | suitable for cell culture |
| suitable for hybridoma | |
| shipped in | dry ice |
| storage temp. | −20°C |
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文献和实验相关专题 胎牛血清产品选择指南 第一类,最高级别,是专门用于干细胞 的特级胎牛血清 1、Hyclone的特级胎牛血清 目前,性能最好的血清,还是要说说Hyclone的特级胎牛血清,货号是SH30070.03,它是Hyclone的“招牌菜”。 Hyclone特级胎牛血清(Defined FBS),货号SH30070,是世界上唯一经过40纳米过滤的顶级胎牛血清,极佳的促细胞生长作用及产品稳定性。内毒
错误常会造成细胞无法存活。 5. 何谓FBS, FCS, CS, HS ? FBS (fetal bovine serum) 和FCS (fetal calf serum) 是相同的意思, 两者都是指胎牛血清, FCS 乃错误的使用字眼, 请不要再使用。CS (calf serum) 则是指小牛血清。HS (horseserum) 则是指马血清。 6. 培养细胞时应使用5 % 或10% CO2?或根本没有影响? 一般培养基中大都使用HCO3-/CO32-/H+ 作为pH
,又考虑是不是血清,我们用的是Hyclone特级胎牛血清,南美血缘的,考虑是不是不如北美血缘的好,又把实验室各种血清试了一遍,结果还是不好,又检查了CO2温箱的温度和湿度是不是稳定。我们把能换的东西都换了一遍,血清,胰酶浓度,培养板,CO2气,最终我们请教了另外的做心肌细胞培养的人,换用胰酶+胶原酶II混合消化的方法,并且每次消化前都轻柔的吹打让组织充分和消化液混合,消化后也充分吹打后静置1-2分钟再吸上清,最后离心完成后,用培养基重悬沉淀是要充分吹打,以避免再下一步过滤时大量的丢失细胞。还要保证种板
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