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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0940 |
---|---|
产品名称 | Human ICAM3 ELISA Kit |
产品描述 | For quantitative detection of human ICAM-3 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Q30-H485, homodimer |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | ICAM3 |
基因全名 | intercellular adhesion molecule 3 |
Uniprot ID | P32942 |
指标别名 | CD50|CD50 antigen|CDW50|ICAM 3|ICAM R|ICAMR|Intercellular adhesion molecule 3|Intercellular adhesion molecule 3 precursor |
背景资料 | Intercellular adhesion molecule 3(ICAM3) also known as CD50(Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0940-CAP | 96-well plate precoated with anti- human ICAM-3 antibody | 1 |
EK0940-ST | Lyophilized recombinant human ICAM-3 standard | 10ng/tube×2 |
EK0940-DA | Biotinylated anti- human ICAM-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 378 | 1191 | 4088 | 362 | 1109 | 4040 |
Standard deviation | 21.92 | 58.35 | 290.24 | 25.7 | 57.66 | 335.32 |
CV(%) | 5.8% | 4.9% | 7.1% | 7.1% | 5.2% | 8.3% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.071 | 0.124 | 0.179 | 0.283 | 0.498 | 0.880 | 1.609 | 2.399 |
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