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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK0765 |
---|---|
产品名称 | Mouse Galectin-3/LGALS3 ELISA Kit |
产品描述 | For quantitative detection of mouse Galectin-3 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, A2-I264 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | LGALS3 |
基因全名 | lectin, galactoside-binding, soluble, 3 |
Uniprot ID | P16110 |
指标别名 | 35 kDa lectin|Carbohydrate-binding protein 35|CBP 35|Gal-3|Galactose-specific lectin 3|Galactoside-binding protein|GALBP|Galectin-3|GALIG|IgE-binding protein|L-31|Laminin-binding protein|Lectin L-29|lectin, galactoside-binding, soluble, 3|LGALS2Mac-2 antigen|MAC2|Macrophage galactose-specific lectin |
背景资料 | Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0765-CAP | 96-well plate precoated with anti- mouse Galectin-3antibody | 1 |
EK0765-ST | Lyophilized recombinant mouse Galectin-3 standard | 10ng/tube×2 |
EK0765-DA | Biotinylated anti- mouse Galectin-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 81 | 434 | 1619 | 80 | 402 | 1701 |
Standard deviation | 6.31 | 31.24 | 126.28 | 7.68 | 32.56 | 153.09 |
CV(%) | 7.8% | 7.2% | 7.8% | 9.6% | 8.1% | 9% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.020 | 0.057 | 0.093 | 0.167 | 0.311 | 0.629 | 1.164 | 1.979 |
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