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发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin).
96T
货号 | EK1257 |
---|---|
产品名称 | Human Ficolin-1 ELISA Kit |
产品描述 | For quantitative detection of human Ficolin-1 in cell culture supernates, serum and plasma(heparin). |
免疫原 | NSO, A30-A326 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <20pg/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | FCN1 |
基因全名 | ficolin (collagen/fibrinogen domain containing) 1 |
Uniprot ID | O00602 |
指标别名 | Collagen/fibrinogen domain containing protein 1|Collagen/fibrinogen domain-containing protein 1|Fcn1|FCN1_HUMAN |FCNM|ficolin (collagen/fibrinogen domain containing) 1|Ficolin 1|Ficolin A|Ficolin alpha|Ficolin-1|Ficolin-A|Ficolin-alpha|M ficolin|M-ficolin |
背景资料 | Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1257-CAP | 96-well plate precoated with anti- human Ficolin-1 antibody | 1 |
EK1257-ST | Lyophilized recombinant human Ficolin-1 standard | 50ng/tube×2 |
EK1257-DA | Biotinylated anti- human Ficolin-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1355 | 8333 | 19299 | 1365 | 8931 | 19839 |
Standard deviation | 88.07 | 483.31 | 1042.14 | 95.55 | 544.79 | 1091.14 |
CV(%) | 6.5% | 5.8% | 5.4% | 7% | 6.1% | 5.5% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.066 | 0.187 | 0.323 | 0.557 | 0.930 | 1.423 | 1.967 | 2.447 |
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