大家都在搜
手机验证
发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1117 |
---|---|
产品名称 | Human Elafin/PI3 ELISA Kit |
产品描述 | For quantitative detection of human Elafin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, A23-Q117 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | PI3 |
基因全名 | peptidase inhibitor 3, skin-derived |
Uniprot ID | P19957 |
指标别名 | Cementoin|Elafin|Elastase specific inhibitor|ESI|MGC13613|Peptidase inhibitor 3|Peptidase inhibitor 3 skin derived|PI 3|PI3|Pre elafin|Protease inhibitor WAP3|SKALP|Skin derived antileukoproteinase|Trappin 2|WAP four disulfide core domain 14|WAP four disulfide core domain protein 14|WAP3|WFDC14 |
背景资料 | Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase(SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core(WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1117-CAP | 96-well plate precoated with anti- human Elafin antibody | 1 |
EK1117-ST | Lyophilized recombinant human Elafin standard | 10ng/tube×2 |
EK1117-DA | Biotinylated anti- human Elafin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 64 | 263 | 893 | 70 | 273 | 891 |
Standard deviation | 4.22 | 12.36 | 51.79 | 4.9 | 15.56 | 61.47 |
CV(%) | 6.6% | 4.7% | 5.8% | 7% | 5.7% | 6.9% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.010 | 0.073 | 0.148 | 0.271 | 0.575 | 0.966 | 1.789 | 2.233 |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。