- ¥80
- 上海钰博
- 进口/国产
- YB246Mu01
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant DNA Methyltransferase 3B (DNMT3B)
- 库存：
10000
- 供应商：
钰博生物
适用生物 Mus musculus (Mouse，小鼠)
DNA甲基转移酶3B(DNMT3B)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 n/a 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
| Organism species | Mus musculus (Mouse) |
| Product No. | RPJ246Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | n/a |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
DNA甲基转移酶3B(DNMT3B)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验; 2)DNM T3a、DNM T3b从头甲基转移酶, 它们可甲基化CPG, 使其半甲基化, 继而全甲基化。从头甲基转移酶可能参与细胞生长分化调控, 其中DNM T3b在肿瘤基因甲基化中起重要作用。三、 相关图解四、 DNMT的抑制剂Decitabine：Decitabine是一种DNA甲基化的有效抑制剂，作用于HL-60和KG1a细胞时，IC50分别为100 ng/mL和1 ng/mL。Lomeguatrib：Lomeguatrib是一种有效的O6-alkylguanine-DNA
生物甲基转移酶N端调节区，故认为DNMT2不具备催化CpG位点甲基化的作用。DNMT3a定位于2p23，DNMT3b定位于20q11．2。
Inhibition of DNA Methylation in Somatic Cells
the methylation marks through cell division. However, the de novo methyltransferases, Dnmt3a and Dnmt3b, can also contribute to the maintenance of the methylation pattern. Manipulation of these enzymes, especially Dnmt1, provides a means to alter DNA methylation
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