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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0809 |
---|---|
产品名称 | Human MCP-4 ELISA Kit |
产品描述 | For quantitative detection of human CCL13 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, Q24-T98 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 7.8pg/ml-500pg/ml |
基因名称 | CCL13 |
基因全名 | chemokine (C-C motif) ligand 13 |
Uniprot ID | Q99616 |
指标别名 | C-C motif chemokine 13|CCL13|CCL13_HUMAN|Chemokine (C C motif) ligand 13|CK beta 10|CK-beta-10|CKb10|Macrophage/monocyte chemotactic protein 4|MCP-4|MGC17134|Monocyte chemoattractant protein 4|Monocyte chemotactic protein 4|NCC 1|NCC-1|NCC1|New CC chemokine 1|SCYA13|SCYL1|short chain|Small inducible cytokine A13|Small inducible cytokine A13 precursor|Small inducible cytokine subfamily A (Cys Cys) member 13|Small-inducible cytokine A13 |
背景资料 | Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0809-CAP | 96-well plate precoated with anti- human CCL13 antibody | 1 |
EK0809-ST | Lyophilized recombinant human CCL13 standard | 10ng/tube×2 |
EK0809-DA | Biotinylated anti- human CCL13 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 19 | 85 | 256 | 18 | 88 | 273 |
Standard deviation | 1.14 | 5.1 | 13.05 | 1.26 | 5.63 | 19.11 |
CV(%) | 6% | 6% | 5.1% | 7% | 6.4% | 7% |
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.004 | 0.025 | 0.054 | 0.116 | 0.254 | 0.636 | 1.469 | 2.245 |
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