- ¥80
- 上海钰博
- 进口/国产
- YB357Hu01
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Cholesterol-25-Hydroxylase (CH25H)
- 库存：
10000
- 供应商：
钰博生物
胆固醇-25-羟化酶(CH25H)重组蛋白
适用生物 Homo sapiens (Human，人)
胆固醇-25-羟化酶(CH25H)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 16.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
yb-6718R Dystrophin肌营养不良蛋白抗体 浓度1mg/ml
yb-3577R Dematin金属离子转运体1抗体 浓度1mg/ml
yb-2162R DKK1抑癌蛋白DKK1抗体 浓度1mg/ml
yb-11549R Dispatched膜转运蛋白DISPA抗体 浓度1mg/ml
yb-13003R DIRAS2DIRAS2蛋白抗体 浓度1mg/ml
yb-0993R DDX58DDX58抗体 浓度1mg/ml
yb-3597R DDX4DDX4抗体 浓度1mg/ml
yb-1672R Duck plague virus鸭瘟病毒DPV抗体 浓度1mg/ml
yb-3514R D-DIMER交联纤维蛋白二聚体抗体 浓度1mg/ml
yb-5835R Drebrin脑发育调节蛋白抗体 浓度1mg/ml
yb-7648R DFFBDNA断裂因子抗体（蛋白酶激活脱氧核糖核酸酶） 浓度1mg/ml
yb-7653R DNase gamma脱氧核糖核酸酶γ抗体 浓度1mg/ml
yb-7659R DATF1死亡相关转录因子1抗体 浓度1mg/ml
yb-7651R DNase I脱氧核糖核酸酶1抗体 浓度1mg/ml
yb-7652R DNase II脱氧核糖核酸酶2抗体 浓度1mg/ml
yb-6800R DAD1细胞死亡防卫蛋白1抗体 浓度1mg/ml
yb-7592R DARCDuffy血型趋化因子受体抗体 浓度1mg/ml
yb-0874R DEKDEK癌基因结合蛋白 浓度1mg/ml
yb-12138R DLGAP1PSD95结合蛋白1抗体 浓度1mg/ml
yb-12139R DLGAP2PSD95结合蛋白2抗体 浓度1mg/ml
yb-12140R DLGAP4PSD95结合蛋白4抗体 浓度1mg/ml
yb-7065R DPF2双PHD-D4锌指蛋白2抗体 浓度1mg/ml
yb-7066R DUSP22双特异性磷酸酶22抗体 浓度1mg/ml
yb-1276R DNAJC10Erdj5/DNAJC10蛋白抗体 浓度1mg/ml
yb-7678R DR6肿瘤细胞调亡素/肿瘤坏死因子受体死亡受体6抗体 浓度1mg/ml
yb-9427R TMIGD1跨膜TMIGD1蛋白抗体 浓度1mg/ml
适用生物 Homo sapiens (Human，人)
胆固醇-25-羟化酶(CH25H)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 16.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Cholesterol-25-Hydroxylase (CH25H)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPG357Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 16.4kDa |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | Trp142~Asn247 (Accession # O95992) with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
胆固醇-25-羟化酶(CH25H)重组蛋白 The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WHLLHHKVP WLYRTFHKVH HQNSSSFALA TQYMSVWELF SLGFFDMMNV TLLGCHPLTT LTFHVVNIWL SVEDHSGYNF PWSTHRLVPF GWYGGVVHHD LHHSHFN
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- WHLLHHKVP WLYRTFHKVH HQNSSSFALA TQYMSVWELF SLGFFDMMNV TLLGCHPLTT LTFHVVNIWL SVEDHSGYNF PWSTHRLVPF GWYGGVVHHD LHHSHFN
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
胆固醇-25-羟化酶(CH25H)重组蛋白 Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-4290R CDC37Hsp90辅助伴侣分子CDC37抗体 浓度1mg/ml
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-6718R Dystrophin肌营养不良蛋白抗体 浓度1mg/ml
yb-3577R Dematin金属离子转运体1抗体 浓度1mg/ml
yb-2162R DKK1抑癌蛋白DKK1抗体 浓度1mg/ml
yb-11549R Dispatched膜转运蛋白DISPA抗体 浓度1mg/ml
yb-13003R DIRAS2DIRAS2蛋白抗体 浓度1mg/ml
yb-0993R DDX58DDX58抗体 浓度1mg/ml
yb-3597R DDX4DDX4抗体 浓度1mg/ml
yb-1672R Duck plague virus鸭瘟病毒DPV抗体 浓度1mg/ml
yb-3514R D-DIMER交联纤维蛋白二聚体抗体 浓度1mg/ml
yb-5835R Drebrin脑发育调节蛋白抗体 浓度1mg/ml
yb-7648R DFFBDNA断裂因子抗体（蛋白酶激活脱氧核糖核酸酶） 浓度1mg/ml
yb-7653R DNase gamma脱氧核糖核酸酶γ抗体 浓度1mg/ml
yb-7659R DATF1死亡相关转录因子1抗体 浓度1mg/ml
yb-7651R DNase I脱氧核糖核酸酶1抗体 浓度1mg/ml
yb-7652R DNase II脱氧核糖核酸酶2抗体 浓度1mg/ml
yb-6800R DAD1细胞死亡防卫蛋白1抗体 浓度1mg/ml
yb-7592R DARCDuffy血型趋化因子受体抗体 浓度1mg/ml
yb-0874R DEKDEK癌基因结合蛋白 浓度1mg/ml
yb-12138R DLGAP1PSD95结合蛋白1抗体 浓度1mg/ml
yb-12139R DLGAP2PSD95结合蛋白2抗体 浓度1mg/ml
yb-12140R DLGAP4PSD95结合蛋白4抗体 浓度1mg/ml
yb-7065R DPF2双PHD-D4锌指蛋白2抗体 浓度1mg/ml
yb-7066R DUSP22双特异性磷酸酶22抗体 浓度1mg/ml
yb-1276R DNAJC10Erdj5/DNAJC10蛋白抗体 浓度1mg/ml
yb-7678R DR6肿瘤细胞调亡素/肿瘤坏死因子受体死亡受体6抗体 浓度1mg/ml
yb-9427R TMIGD1跨膜TMIGD1蛋白抗体 浓度1mg/ml
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文献和实验相关实验
亦能生存。然而野生株和突变株均不能在碱性环境中生长繁殖。存在野生株和尿素的酸性溶液的pH迅速从3.5升至8.45。尿素酶突变株在pH4.5～7.2的溶液中存活，不对尿素起反应。野生株N6暴露于氨的浓度高达80mmol仍能存活。胃的酸性环境可能对存活于尿素中的Hp有杀伤作用。但是Hp决不能存活于含有尿素的正常环境中，因为随后升高的pH的危害性远超过氨的毒性。 在血琼脂中，Hp不能在含有5％胆汁的情况下生长。但是暴露于含有5％胆汁的液体培养基中30min，只有25％的Hp被杀死。这说明Hp
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胆固醇-25-羟化酶(CH25H)重组蛋白
¥80
