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血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等
48T/96T
规格: | 48T | 产品价格: | ¥1080.0 |
---|---|---|---|
规格: | 96T | 产品价格: | ¥1896.0 |
人雄激素受体(AR)ELISA试剂盒
酶联免疫吸附法
酶联免疫吸附法(Enzyme-Linked Immunosorbent Assay,ELISA)是一种灵敏、特异的免疫学技术,用于检测抗原或抗体的存在。其基本原理是将抗原或抗体与酶结合,形成酶标抗原或抗体,保留其免疫活性,同时又保留酶的活性。然后将酶标抗原或抗体与固相载体表面结合,形成酶-抗原-抗体复合物。最后通过底物显色反应,根据颜色变化来检测抗原或抗体的存在。ELISA具有高灵敏度、高特异性、快速、易操作等优点,在医学、生物领域得到广泛应用。
试剂盒性能
检测范围:5 pg/mL – 160 pg/mL。
灵敏度:检测浓度小于1.0 pg/mL。
特异性:不与其它可溶性结构类似物交叉反应。
重复性:板内变异系数小于10% ,板间变异系数小于15% 。
实验步骤
1. 准备试剂盒:将试剂盒从冰箱中取出,室温放置30分钟以上,使试剂盒恢复至室温。
2. 加样:分别向各孔中加入标准品、待测样本和空白孔,每孔加入50μl。
3. 孵育:用封板胶纸封住反应孔,轻轻振荡混匀,37℃孵育1小时。
4. 洗涤:甩去孔内液体,用洗涤液充分洗涤4-5次,每次30秒。
5. 加酶标二抗:每孔加入50μl酶标二抗溶液,轻轻振荡混匀,37℃孵育30分钟。
6. 洗涤:甩去孔内液体,用洗涤液充分洗涤4-5次,每次30秒。
7. 加底物:每孔加入50μl底物溶液,轻轻振荡混匀,37℃孵育15-20分钟。
8. 终止反应:每孔加入50μl终止液,轻轻振荡混匀。
9. 读数:用酶标仪在450nm波长处读取各孔的光密度值。
操作注意事项
1. 试剂盒保存在 2-8℃,使用前室温平衡 20 分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶溶解后再使用。
2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。
3. 浓度为 0 的标准品可视为阴性对照或者空白;按照说明书操作时样本已经稀释 5 倍,终结果乘以5才是样本终浓度。
4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。
5. 所有液体组分使用前充分摇匀。
6. 若中英文说明书有误,请以中文说明书为准。
7. 20×洗涤缓冲液的稀释:蒸馏水按 1:20 稀释,即 1 份的 20×洗涤缓冲液加 19 份的蒸馏水。
8. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
人雄激素受体(AR)ELISA试剂盒
洗板方法
1.手工洗板:甩尽孔内液体,每孔加满洗涤液,静置 1min 后甩尽孔内液体,在吸水纸上拍干,如此洗板 5次。
2.自动洗板机:每孔注入洗液 350μL,浸泡 1min,洗板 5 次。
结果判断:
1. 每个标准品的OD值减去空白孔的OD值后作图,如设置复孔,则应取其平均值计算。以标准品的浓度为横坐标,OD值为纵坐标,绘出标准曲线。亦可以OD值为横坐标,标准品的浓度为纵坐标,绘出标准曲线。
2. 推荐使用专业的曲线制作软件,如curve expert 1.3或1.4,在软件界面既可根据样品OD值,由标准曲线查出相应的浓度,乘以稀释倍数;亦可将样品的OD值代入标准曲线的拟合方程式,计算出样品浓度,再乘以稀释倍数,即为样品的实际浓度。
3. 若样品OD值高于标准曲线上限,应适当稀释后重测,计算浓度时应乘以稀释倍数。
人雄激素受体(AR)ELISA试剂盒
ELISA试剂盒的作用
ELISA试剂盒是一种用于体外定性检测人血清或血浆中的特定抗体的试剂盒,例如抗人类戊型肝炎(HEV)病毒IgM抗体ELISA检测试剂盒。
ELISA试剂盒基于抗原抗体之间的特异结合反应,以前用于免疫测定的抗原通常为各种纯化抗原,而抗体则为纯化抗原免疫动物后获得的多克隆抗体和使用杂交瘤技术得到的单克隆抗体,而抗原或抗体的标记物如酶标结合物则通过各种化学合成方法制备。
ELISA试剂盒具有灵敏度高、特异性好、操作简便、安全、快速等优点,可以用于检测类风湿因子、病毒、细菌等,在临床诊断、科研和生物制品检测等领域具有广泛的应用。
人雄激素受体(AR)ELISA试剂盒
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DM6255 | 人氧固醇结合蛋白样蛋白1A(OSBPL1A)酶联免疫试剂盒 | Human OSBPL1A (Oxysterol Binding Protein Like Protein 1A) ELISA Kit |
DM6256 | 人饥饿素(GHRL)酶联免疫试剂盒 | Human GHRL (Ghrelin) ELISA Kit |
DM6257 | 人精氨酸酶(ARG2)酶联免疫试剂盒 | Human ARG2 (Arginase II) ELISA Kit |
DM6258 | 人生长激素诱导跨膜蛋白(GHITM)酶联免疫试剂盒 | Human GHITM (Growth Hormone Inducible Transmembrane Protein) ELISA Kit |
DM6259 | 人生长相关蛋白43(GAP43)酶联免疫试剂盒 | Human GAP43 (Growth Associated Protein 43) ELISA Kit |
DM6260 | 人生长抑素(SST)酶联免疫试剂盒 | Human SST (Somatostatin) ELISA Kit |
DM6261 | 人生长抑素受体1(SSTR1)酶联免疫试剂盒 | Human SSTR1 (Somatostatin Receptor 1) ELISA Kit |
DM6262 | 人生长因子受体结合蛋白10(Grb10)酶联免疫试剂盒 | Human Grb10 (Growth Factor Receptor Bound Protein 10) ELISA Kit |
DM6263 | 人生长因子受体结合蛋白14(Grb14)酶联免疫试剂盒 | Human Grb14 (Growth Factor Receptor Bound Protein 14) ELISA Kit |
DM6264 | 人生长因子受体结合蛋白2(Grb2)酶联免疫试剂盒 | Human Grb2 (Growth Factor Receptor Bound Protein 2) ELISA Kit |
DM6265 | 人生长因子受体结合蛋白7(Grb7)酶联免疫试剂盒 | Human Grb7 (Growth Factor Receptor Bound Protein 7) ELISA Kit |
DM6266 | 人食欲素B/阿立新B(OXB)酶联免疫试剂盒 | Human OXB (Orexin B) ELISA Kit |
DM6267 | 人视黄酸诱导基因/RIG-1样受体(RLR)酶联免疫试剂盒 | Human RLR (Retinoic Acid Inducible Gene/RIG-Like Receptor) ELISA Kit |
DM6268 | 人视网膜母细胞瘤蛋白1(RB1)酶联免疫试剂盒 | Human RB1 (Retinoblastoma Protein 1) ELISA Kit |
DM6269 | 人视网膜母细胞瘤抑制蛋白(PRB)酶联免疫试剂盒 | Human PRB (Retinoblastoma Tumor Suppressor Protein) ELISA Kit |
DM6270 | 人嗜环蛋白/亲环素A(CYPA)酶联免疫试剂盒 | Human CYPA (Cyclophilin A) ELISA Kit |
DM6271 | 人嗜环蛋白/亲环素B(CYPB)酶联免疫试剂盒 | Human CYPB (Cyclophilin B) ELISA Kit |
DM6272 | 人嗜环蛋白/亲环素D(CYPD)酶联免疫试剂盒 | Human CYPD (Cyclophilin D) ELISA Kit |
DM6273 | 人神经纤维网蛋白2(NRP2)酶联免疫试剂盒 | Human NRP2 (Neuropilin 2) ELISA Kit |
DM6274 | 人柠檬酸合酶(CS)酶联免疫试剂盒 | Human CS (Citrate Synthase) ELISA Kit |
DM6275 | 人8-羟基鸟嘌呤糖苷酶1(OGG1)酶联免疫试剂盒 | Human OGG1 (8-Oxoguanine Glycosylase 1) ELISA Kit |
DM6276 | 人嗜酸粒细胞趋化因子受体(ECFR)酶联免疫试剂盒 | Human ECFR (Eosinophil Chemotactic Factor Receptor) ELISA Kit |
DM6277 | 人嗜酸性白血球相关之RNA水解酵素家族成员1(EAR1)酶联免疫试剂盒 | Human EAR1 (Eosinophil Associated Ribonuclease A family Member 1) ELISA Kit |
DM6278 | 人嗜酸性白血球相关之RNA水解酵素家族成员12(EAR12)酶联免疫试剂盒 | Human EAR12 (Eosinophil Associated Ribonuclease A family Member 12) ELISA Kit |
DM6279 | 人嗜酸性白血球相关之RNA水解酵素家族成员2(EAR2)酶联免疫试剂盒 | Human EAR2 (Eosinophil Associated Ribonuclease A family Member 2) ELISA Kit |
DM6280 | 人嗜酸性白血球相关之RNA水解酵素家族成员3(EAR3)酶联免疫试剂盒 | Human EAR3 (Eosinophil Associated Ribonuclease A family Member 3) ELISA Kit |
DM6281 | 人头蛋白(NOG)酶联免疫试剂盒 | Human NOG (Noggin) ELISA Kit |
DM6282 | 人中性粒细胞弹性蛋白酶(NE/ELA2)酶联免疫试剂盒 | Human NE/ELA2 (Elastase 2, Neutrophil) ELISA Kit |
DM6283 | 人受磷蛋白(PLN)酶联免疫试剂盒 | Human PLN (Phospholamban) ELISA Kit |
DM6284 | 人受体TNFRSF结合丝氨酸苏氨酸激酶2(RIPK2)酶联免疫试剂盒 | Human RIPK2 (Receptor TNFRSF Interacting Serine Threonine Kinase 2) ELISA Kit |
DM6285 | 人非受体酪氨酸激酶2(TNK2)酶联免疫试剂盒 | Human TNK2 (Tyrosine Kinase, Non Receptor 2) ELISA Kit |
DM6286 | 人衰变加速因子(DAF)酶联免疫试剂盒 | Human DAF (Decay Accelerating Factor) ELISA Kit |
DM6287 | 人丝氨酸/苏氨酸激酶39(STK39)酶联免疫试剂盒 | Human STK39 (Serine/Threonine Kinase 39) ELISA Kit |
DM6288 | 人丝氨酸蛋白酶(PRSS)酶联免疫试剂盒 | Human PRSS (Serine Protease) ELISA Kit |
DM6289 | 人丝氨酸/苏氨酸激酶TNNI3K(TNNI3K)酶联免疫试剂盒 | Human TNNI3K (Serine/threonine-protein kinase TNNI3K) ELISA Kit |
DM6290 | 人丝裂原激活蛋白激酶激酶6(MAP2K6/MKK6)酶联免疫试剂盒 | Human MAP2K6/MKK6 (Mitogen Activated Protein Kinase Kinase 6) ELISA Kit |
DM6291 | 人丝裂原激活蛋白激酶(MAPK)酶联免疫试剂盒 | Human MAPK (Mitogen Activated Protein Kinase) ELISA Kit |
DM6292 | 人丝状血球凝集素(FHA)酶联免疫试剂盒 | Human FHA (Filamentous Hemagglutinin) ELISA Kit |
DM6293 | 人死亡相关蛋白6(DAP6)酶联免疫试剂盒 | Human DAP6 (Death Associated Protein 6) ELISA Kit |
DM6294 | 人松弛素/胰岛素样肽受体1(RXFP1)酶联免疫试剂盒 | Human RXFP1 (Relaxin/Insulin Like Family Peptide Receptor 1) ELISA Kit |
DM6295 | 人速激肽受体1(TACR1)酶联免疫试剂盒 | Human TACR1 (Tachykinin Receptor 1) ELISA Kit |
DM6296 | 人速激肽受体2(TACR2)酶联免疫试剂盒 | Human TACR2 (Tachykinin Receptor 2) ELISA Kit |
DM6297 | 人速激肽受体3(TACR3)酶联免疫试剂盒 | Human TACR3 (Tachykinin Receptor 3) ELISA Kit |
DM6298 | 人髓过氧化物酶抗体(Anti-MPO)酶联免疫试剂盒 | Human Anti-MPO (Anti-Myeloperoxidase Antibody) ELISA Kit |
DM6299 | 人鞘磷脂脂质蛋白1(PLP1)酶联免疫试剂盒 | Human PLP1 (Proteolipid Protein 1, Myelin) ELISA Kit |
DM6300 | 人髓分化因子88(MyD88)酶联免疫试剂盒 | Human MyD88 (Myeloid Differentiation Factor 88) ELISA Kit |
DM6301 | 人端粒保护蛋白1(POT1)酶联免疫试剂盒 | Human POT1 (Protection of Telomeres Protein 1) ELISA Kit |
DM6302 | 人胎儿血红蛋白(HBF)酶联免疫试剂盒 | Human HBF (Fetal Hemoglobin) ELISA Kit |
DM6303 | 人蛋白酶3(PR3)酶联免疫试剂盒 | Human PR3 (Proteinase 3) ELISA Kit |
DM6304 | 人胎盘催乳素(PL)酶联免疫试剂盒 | Human PL (Placental Lactogen) ELISA Kit |
DM6305 | 人胎盘蛋白(PP)酶联免疫试剂盒 | Human PP (Placental Protein) ELISA Kit |
DM6306 | 人胎盘蛋白13 (PP13)酶联免疫试剂盒 | Human PP13(Placental Protein13)ELISA Kit |
DM6307 | 人胎盘蛋白14 (PP14)酶联免疫试剂盒 | Human PP14 (Placental Protein14) ELISA Kit |
DM6308 | 人胎盘核糖核酸抑制剂(PRI)酶联免疫试剂盒 | Human PRI (Placental Ribonuclease Inhibitor) ELISA Kit |
DM6309 | 人胎盘碱性磷酸酶(PLAP/ALPP)酶联免疫试剂盒 | Human PLAP/ALPP(Placental Alkaline Phosphatase)ELISA Kit |
DM6310 | 人肉毒碱棕榈酰基转移酶1A(CPT1A)酶联免疫试剂盒 | Human CPT1A (Carnitine Palmitoyltransferase 1A, Liver) ELISA Kit |
DM6311 | 人肉毒碱棕榈酰基转移酶1B(CPT1B)酶联免疫试剂盒 | Human CPT1B (Carnitine Palmitoyltransferase 1B, Muscle) ELISA Kit |
DM6312 | 人胎球蛋白B(FETUB)酶联免疫试剂盒 | Human FETUB (Fetuin B) ELISA Kit |
DM6313 | 人氧固醇结合蛋白样蛋白8(OSBPL8)酶联免疫试剂盒 | Human OSBPL8 (Oxysterol Binding Protein Like Protein 8) ELISA Kit |
DM6314 | 人补体因子H相关蛋白3(CFHR3)酶联免疫试剂盒 | Human CFHR3 (Complement Factor H Related Protein 3) ELISA Kit |
DM6315 | 人肽聚糖识别蛋白S(PGRP-S)酶联免疫试剂盒 | Human PGRP-S (Peptidoglycan Recognition Protein-S) ELISA Kit |
DM6316 | 人肽酰脯氨酰异构酶/亲环素样蛋白1(PPIL1)酶联免疫试剂盒 | Human PPIL1 (Peptidylprolyl Isomerase/Cyclophilin Like 1) ELISA Kit |
DM6317 | 人肥胖抑制素(OB)酶联免疫试剂盒 | Human OB (Obestatin) ELISA Kit |
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