- 询价
- 上海钰博
- 进口/国产
- YB888Po01
- 2025年07月13日
13 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Chromogranin B (CHGB)
- 库存：
10000
- 供应商：
钰博生物
嗜铬蛋白B(CHGB)重组蛋白
适用生物 Sus scrofa; Porcine (Pig，猪)
嗜铬蛋白B(CHGB)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 58.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybD071Hu01 碳酸酐酶Ⅳ(CA4)重组蛋白 Recombinant Carbonic Anhydrase IV (CA4) Homo sapiens (Human)
ybD072Hu01 碳酸酐酶ⅤB(CA5B)重组蛋白 Recombinant Carbonic Anhydrase VB, Mitochondrial (CA5B) Homo sapiens (Human)
ybD073Hu01 碳酸酐酶Ⅵ(CA6)重组蛋白 Recombinant Carbonic Anhydrase VI (CA6) Homo sapiens (Human)
ybD076Ra01 碳酸酐酶Ⅸ(CA9)重组蛋白 Recombinant Carbonic Anhydrase IX (CA9) Rattus norvegicus (Rat)
ybD085Hu01 花生四烯酸脂加氧酶3(ALOXE3)重组蛋白 Recombinant Arachidonate Lipoybgenase 3 (ALOXE3) Homo sapiens (Human)
ybD089Mu01 溶酶体酸性磷酸酶2(ACP2)重组蛋白 Recombinant Acid Phosphatase 2, Lysosomal (ACP2) Mus musculus (Mouse)
ybD093Hu01 血红蛋白γ1(HBγ1)重组蛋白 Recombinant Hemoglobin Gamma 1 (HBg1) Homo sapiens (Human)
ybD095Hu01 血红蛋白γ2(HBγ2)重组蛋白 Recombinant Hemoglobin Gamma 2 (HBg2) Homo sapiens (Human)
ybD098Hu01 血红蛋白β(HBβ)重组蛋白 Recombinant Hemoglobin Beta (HBb) Homo sapiens (Human)
ybD101Hu01 低密度脂蛋白受体相关蛋白2(LRP2)重组蛋白 Recombinant Low Density Lipoprotein Receptor Related Protein 2 (LRP2) Homo sapiens (Human)
ybD105Hu01 低密度脂蛋白受体相关蛋白6(LRP6)重组蛋白 Recombinant Low Density Lipoprotein Receptor Related Protein 6 (LRP6) Homo sapiens (Human)
ybD108Hu01 激肽释放酶2(KLK2)重组蛋白 Recombinant Kallikrein 2 (KLK2) Homo sapiens (Human)
ybD111Hu01 胃内因子(GIF)重组蛋白 Recombinant Gastric Intrinsic Factor (GIF) Homo sapiens (Human)
ybD115Hu01 沙漠刺猬因子(DHH)重组蛋白 Recombinant Hedgehog Homolog, Desert (DHH) Homo sapiens (Human)
ybD116Hu01 印度刺猬因子(IHH)重组蛋白 Recombinant Hedgehog Homolog, Indian (IHH) Homo sapiens (Human)
ybD133Hu01 ⅩⅣ型胶原(COL14)重组蛋白 Recombinant Collagen Type XIV (COL14) Homo sapiens (Human)
ybD134Hu01 白介素1η(IL1η)重组蛋白 Recombinant Interleukin 1 Eta (IL1h) Homo sapiens (Human)
ybD140Hu01 尿皮质素3(UCN3)重组蛋白 Recombinant Urocortin 3 (UCN3) Homo sapiens (Human)
适用生物 Sus scrofa; Porcine (Pig，猪)
嗜铬蛋白B(CHGB)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 58.4kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Chromogranin B (CHGB)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Sus scrofa; Porcine (Pig) |
| Product No. | RPC888Po01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 58.4kDa |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | Val40~His326 (Accession # Q9GLG4) with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-V LSNALSKSNA PPITPECRQV LKKGGKEVKD EEKGANENTR FEVRLLRDPA DTSETQRPSG GREGAEAPSE DTQGPPGADVEGGGHSREGA GKPRGGPYSS DNPVAKEGKT RHSEKSEGQD REEEEGEKYQ KRERGEEGSE ERHLQEPGET QTAFLNQGNR ATAKKKEEFE SRYDAHSAGG PAKTHSRERS SQESGEETGSQDAAPGEPES PPEGQEAPEE SQEDASLEVD KRRWRPRHHH GRSRPDRAPQ EGSPPPEERG HPREESEESV LGLASPGGQR TRHPTH
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-V LSNALSKSNA PPITPECRQV LKKGGKEVKD EEKGANENTR FEVRLLRDPA DTSETQRPSG GREGAEAPSE DTQGPPGADVEGGGHSREGA GKPRGGPYSS DNPVAKEGKT RHSEKSEGQD REEEEGEKYQ KRERGEEGSE ERHLQEPGET QTAFLNQGNR ATAKKKEEFE SRYDAHSAGG PAKTHSRERS SQESGEETGSQDAAPGEPES PPEGQEAPEE SQEDASLEVD KRRWRPRHHH GRSRPDRAPQ EGSPPPEERG HPREESEESV LGLASPGGQR TRHPTH
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
嗜铬蛋白B(CHGB)重组蛋白 Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
嗜铬蛋白B(CHGB)重组蛋白 Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybD070Hu01 碳酸酐酶Ⅲ(CA3)重组蛋白 Recombinant Carbonic Anhydrase III, Muscle Specific (CA3) Homo sapiens (Human)
嗜铬蛋白B(CHGB)重组蛋白 Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybD071Hu01 碳酸酐酶Ⅳ(CA4)重组蛋白 Recombinant Carbonic Anhydrase IV (CA4) Homo sapiens (Human)
ybD072Hu01 碳酸酐酶ⅤB(CA5B)重组蛋白 Recombinant Carbonic Anhydrase VB, Mitochondrial (CA5B) Homo sapiens (Human)
ybD073Hu01 碳酸酐酶Ⅵ(CA6)重组蛋白 Recombinant Carbonic Anhydrase VI (CA6) Homo sapiens (Human)
ybD076Ra01 碳酸酐酶Ⅸ(CA9)重组蛋白 Recombinant Carbonic Anhydrase IX (CA9) Rattus norvegicus (Rat)
ybD085Hu01 花生四烯酸脂加氧酶3(ALOXE3)重组蛋白 Recombinant Arachidonate Lipoybgenase 3 (ALOXE3) Homo sapiens (Human)
ybD089Mu01 溶酶体酸性磷酸酶2(ACP2)重组蛋白 Recombinant Acid Phosphatase 2, Lysosomal (ACP2) Mus musculus (Mouse)
ybD093Hu01 血红蛋白γ1(HBγ1)重组蛋白 Recombinant Hemoglobin Gamma 1 (HBg1) Homo sapiens (Human)
ybD095Hu01 血红蛋白γ2(HBγ2)重组蛋白 Recombinant Hemoglobin Gamma 2 (HBg2) Homo sapiens (Human)
ybD098Hu01 血红蛋白β(HBβ)重组蛋白 Recombinant Hemoglobin Beta (HBb) Homo sapiens (Human)
ybD101Hu01 低密度脂蛋白受体相关蛋白2(LRP2)重组蛋白 Recombinant Low Density Lipoprotein Receptor Related Protein 2 (LRP2) Homo sapiens (Human)
ybD105Hu01 低密度脂蛋白受体相关蛋白6(LRP6)重组蛋白 Recombinant Low Density Lipoprotein Receptor Related Protein 6 (LRP6) Homo sapiens (Human)
ybD108Hu01 激肽释放酶2(KLK2)重组蛋白 Recombinant Kallikrein 2 (KLK2) Homo sapiens (Human)
ybD111Hu01 胃内因子(GIF)重组蛋白 Recombinant Gastric Intrinsic Factor (GIF) Homo sapiens (Human)
ybD115Hu01 沙漠刺猬因子(DHH)重组蛋白 Recombinant Hedgehog Homolog, Desert (DHH) Homo sapiens (Human)
ybD116Hu01 印度刺猬因子(IHH)重组蛋白 Recombinant Hedgehog Homolog, Indian (IHH) Homo sapiens (Human)
ybD133Hu01 ⅩⅣ型胶原(COL14)重组蛋白 Recombinant Collagen Type XIV (COL14) Homo sapiens (Human)
ybD134Hu01 白介素1η(IL1η)重组蛋白 Recombinant Interleukin 1 Eta (IL1h) Homo sapiens (Human)
ybD140Hu01 尿皮质素3(UCN3)重组蛋白 Recombinant Urocortin 3 (UCN3) Homo sapiens (Human)
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
重组蛋白纯化基本原则蛋白的分离纯化是生物工程下游阶段一个比较重要的部分，尤其在基因工程重组蛋白的分离纯化中，上游过程的许多因素会直接影响到下游蛋白的分离，充分利用上游对下游的影响，对蛋白的纯化做一个全面的考虑和整体的设计。是否带有亲和标签，His标签，GST标签等，不同的亲和标签选择不同的纯化方案；可能是可溶性表达，可能形成包涵体，可溶性的蛋白往往需要复杂的纯化步骤，而包涵体易于分离，纯度较高，但回收具有生物活性的蛋白却变的相当困难，需要对聚集的蛋白进行变复性，通常活性蛋白的得率比较低；是否
液稀释。 2. 一定要溶解到指定的浓度。 蛋白在一定的浓度范围内可以保持良好的稳定性，这个范围就是说明书上所标明的浓度范围，该例为0.1-1.0 mg/ml。这个浓度范围对于不同的细胞因子或重组蛋白是不同的。如重组人FGF-6(产品编号：100-30)为0.5-1.0 mg/ml，而重组人Activin B(产品编号：120-15)则一定要是0.5 mg/ml。 问：高于或低于该浓度范围会有什么问题呢? 答：首先，细胞因子或蛋白会不稳定，即很容易出现活性
范围，该例为0.1-1.0 mg/ml。这个浓度范围对于不同的细胞因子或重组蛋白是不同的。如重组人FGF-6(产品编号：100-30)为0.5-1.0mg/ml，而重组人Activin B(产品编号：120-15)则一定要是0.5mg/ml。 高于或低于该浓度范围会有什么问题呢？ 答：首先，细胞因子或蛋白会不稳定，即很容易出现活性下降的现象。其次，高于这个浓度范围，可能会超过该蛋白的最大溶解浓度，即蛋白无法完全溶解。再者，高于或低于该浓度范围，蛋白可能会出现聚集(aggregation
技术资料暂无技术资料 索取技术资料
嗜铬蛋白B(CHGB)重组蛋白
询价
