- ¥80
- 上海钰博
- 进口/国产
- YB323Hu01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)
- 库存：
10000
- 供应商：
钰博生物
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重组蛋白
适用生物 Homo sapiens (Human，人)
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 35.0kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
yb-4937R Cystatin A胱抑素A/半胱氨酸蛋白酶抑制剂A抗体 浓度1mg/ml
yb-15289R C8orf48号染色体开放阅读框4抗体 浓度1mg/ml
yb-4834R CD73CD73抗体 浓度1mg/ml
yb-11074R CNTN4/AXCAM轴突相关粘附分子抗体 浓度1mg/ml
yb-11047R COL6A56型胶原蛋白α5抗体 浓度1mg/ml
yb-11046R CHL1神经细胞粘附分子CALL抗体 浓度1mg/ml
yb-9892R CAP57中性粒细胞杀菌蛋白BP30抗体 浓度1mg/ml
yb-9974R C21ORF5621号染色体开放阅读框56抗体 浓度1mg/ml
yb-4961R CD1AT淋巴细胞CD1抗体 浓度1mg/ml
yb-1440R Cytokeratin 5细胞角蛋白5抗体 浓度1mg/ml
yb-11190R Complement component C9a补体C9a抗体 浓度1mg/ml
yb-9690R C20ORF14120号染色体开放阅读框141抗体 浓度1mg/ml
yb-9695R C20orf16020号染色体开放阅读框160抗体 浓度1mg/ml
yb-9878R Cyclophilin 40亲环蛋白（亲环素）40抗体 浓度1mg/ml
yb-4957R CRTAMT细胞调控相关蛋白抗体 浓度1mg/ml
yb-11021R CRIPT突触后蛋白CRIPT抗体 浓度1mg/ml
yb-11077R Cadherin 8钙粘附蛋白8抗体 浓度1mg/ml
yb-9538R C6orf1746号染色体开放阅读框174抗体 浓度1mg/ml
yb-9537R C6orf1506号染色体开放阅读框150抗体 浓度1mg/ml
yb-9550R CXorf56X染色体开放阅读框56抗体 浓度1mg/ml
yb-9497R C9orf1529号染色体开放阅读框152抗体 浓度1mg/ml
yb-9540R C6orf586号染色体开放阅读框58抗体 浓度1mg/ml
yb-11076R CNTNAP4接触蛋白相关蛋白4抗体 浓度1mg/ml
yb-4891R CD24CD24抗体 浓度1mg/ml
适用生物 Homo sapiens (Human，人)
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 35.0kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPC323Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 35.0kDa |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Arg637~Lys905 (Accession # Q13822) with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RLHT KGSTEERHLL YGRPAVLYRT RYDILYHTDF ESGYSEIFLM PLWTSYTVSK QAEVSSVPDH LTSCVRPDVR VSPSFSQNCL AYKNDKQMSY GFLFPPYLSS SPEAKYDAFL VTNMVPMYPA FKRVWNYFQR VLVKKYASER NGVNVISGPI FDYDYDGLHD TEDKIKQYVE GSSIPVPTHY YSIITSCLDF TQPADKCDGP LSVSSFILPH RPDNEESCNS SEDESKWVEE LMKMHTARVR DIEHLTSLDF FRKTSRSYPE ILTLK
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RLHT KGSTEERHLL YGRPAVLYRT RYDILYHTDF ESGYSEIFLM PLWTSYTVSK QAEVSSVPDH LTSCVRPDVR VSPSFSQNCL AYKNDKQMSY GFLFPPYLSS SPEAKYDAFL VTNMVPMYPA FKRVWNYFQR VLVKKYASER NGVNVISGPI FDYDYDGLHD TEDKIKQYVE GSSIPVPTHY YSIITSCLDF TQPADKCDGP LSVSSFILPH RPDNEESCNS SEDESKWVEE LMKMHTARVR DIEHLTSLDF FRKTSRSYPE ILTLK
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重组蛋白 Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-9979R C21orf7021号染色体开放阅读框70抗体 浓度1mg/ml
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-4937R Cystatin A胱抑素A/半胱氨酸蛋白酶抑制剂A抗体 浓度1mg/ml
yb-15289R C8orf48号染色体开放阅读框4抗体 浓度1mg/ml
yb-4834R CD73CD73抗体 浓度1mg/ml
yb-11074R CNTN4/AXCAM轴突相关粘附分子抗体 浓度1mg/ml
yb-11047R COL6A56型胶原蛋白α5抗体 浓度1mg/ml
yb-11046R CHL1神经细胞粘附分子CALL抗体 浓度1mg/ml
yb-9892R CAP57中性粒细胞杀菌蛋白BP30抗体 浓度1mg/ml
yb-9974R C21ORF5621号染色体开放阅读框56抗体 浓度1mg/ml
yb-4961R CD1AT淋巴细胞CD1抗体 浓度1mg/ml
yb-1440R Cytokeratin 5细胞角蛋白5抗体 浓度1mg/ml
yb-11190R Complement component C9a补体C9a抗体 浓度1mg/ml
yb-9690R C20ORF14120号染色体开放阅读框141抗体 浓度1mg/ml
yb-9695R C20orf16020号染色体开放阅读框160抗体 浓度1mg/ml
yb-9878R Cyclophilin 40亲环蛋白（亲环素）40抗体 浓度1mg/ml
yb-4957R CRTAMT细胞调控相关蛋白抗体 浓度1mg/ml
yb-11021R CRIPT突触后蛋白CRIPT抗体 浓度1mg/ml
yb-11077R Cadherin 8钙粘附蛋白8抗体 浓度1mg/ml
yb-9538R C6orf1746号染色体开放阅读框174抗体 浓度1mg/ml
yb-9537R C6orf1506号染色体开放阅读框150抗体 浓度1mg/ml
yb-9550R CXorf56X染色体开放阅读框56抗体 浓度1mg/ml
yb-9497R C9orf1529号染色体开放阅读框152抗体 浓度1mg/ml
yb-9540R C6orf586号染色体开放阅读框58抗体 浓度1mg/ml
yb-11076R CNTNAP4接触蛋白相关蛋白4抗体 浓度1mg/ml
yb-4891R CD24CD24抗体 浓度1mg/ml
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文献和实验相关实验
，你也可以选择用NdeⅠ直接从 起始密码子后插入外源片断，或者在得到表达产物后利用蛋白氨基酸 的酶切位点把多余的多肽切除。在重组技术上，Novagen除了传统的酶切、连 接方式外，还有不需要连接的克隆方式：Ligation-Independent cloning，简称LIC。采用LIC方法的pET载体是线性化的，在末端有12-15个突出的碱基以在退火时和目的片断互补。在设计PCR扩增引物时 加入与LIC载体互补的序列，PCR产物用3’→5’的内切酶消化出单链与载体互补的序列。通过这种方式
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外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)重组蛋白
¥80
