- ¥80
- 上海钰博
- 进口/国产
- YB056Hu01
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Matrix Metalloproteinase 14 (MMP14)
- 库存：
10000
- 供应商：
钰博生物
基质金属蛋白酶14(MMP14)重组蛋白
适用生物 Homo sapiens (Human，人)
基质金属蛋白酶14(MMP14)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 25.2kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
yb-7975R ATXN7L1共济失调蛋白7样1抗体 浓度1mg/ml
yb-6915R AUTS2孤独症易感基因2蛋白抗体（自闭症相关蛋白1） 浓度1mg/ml
yb-7974R ATX2脊髓小脑共济失调2型蛋白抗体 浓度1mg/ml
yb-9101R ARSH芳香基硫酸酯酶H抗体 浓度1mg/ml
yb-6476R ACF1溴区结构域相邻锌指蛋白1A抗体 浓度1mg/ml
yb-6477R Apo-1载脂蛋白1抗体 浓度1mg/ml
yb-11039R Amisyn突触融合结合蛋白6抗体 浓度1mg/ml
yb-8585R Arginase 1精氨酸酶1抗体 浓度1mg/ml
yb-9532R AKD1腺苷酸激酶结构域蛋白1抗体 浓度1mg/ml
yb-11180R ABCA7三磷酸腺苷结合盒转运蛋白7抗体 浓度1mg/ml
yb-8629R phospho-Na+/K+-ATPase Alpha1(Ser 943)磷酸化钠钾ATP酶α1多肽抗体 浓度1mg/ml
yb-9570R ATP1A1Na+/K+-ATPase α1 钠钾ATP酶α1抗体 浓度1mg/ml
yb-11195R BAF53b肌动蛋白样蛋白6B抗体 浓度1mg/ml
yb-11318R Ataxin 7脊髓小脑共济失调蛋白7抗体 浓度1mg/ml
yb-11329R phospho-c-Abl (Tyr70)磷酸化非受体酪氨酸激酶c-Abl抗体 浓度1mg/ml
yb-11334R APBA1β淀粉样前体蛋白结合蛋白1抗体（X11α） 浓度1mg/ml
yb-4892R Apolipoprotein E载脂蛋白E抗体 浓度1mg/ml
yb-4821R AFP甲胎蛋白AFP抗体 浓度1mg/ml
yb-9847R AE2阴离子交换蛋白2抗体 浓度1mg/ml
yb-9848R Aconitase 1铁调节蛋白1抗体 浓度1mg/ml
yb-9108R ATAD3A三磷酸腺苷酶家族蛋白3A抗体 浓度1mg/ml
yb-1354R APOJ载脂蛋白J抗体 浓度1mg/ml
yb-1570R APOH载脂蛋白H抗体 浓度1mg/ml
yb-7621R ART4二磷酸腺苷核糖基转移酶4抗体 浓度1mg/ml
yb-9082R AKIRIN2AKIRIN2蛋白抗体 浓度1mg/ml
yb-9304R ARL8BADP核糖基化样因子8B抗体 浓度1mg/ml
yb-9110R ATAD2三磷酸腺苷酶家族蛋白2抗体 浓度1mg/ml
yb-9081R AKIRIN1AKIRIN1蛋白抗体 浓度1mg/ml
yb-9325R ASZ1锚定蛋白样蛋白1抗体 浓度1mg/ml
yb-6875R Adenylate kinase 2腺苷酸激酶2抗体 浓度1mg/ml
适用生物 Homo sapiens (Human，人)
基质金属蛋白酶14(MMP14)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 25.2kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Matrix Metalloproteinase 14 (MMP14)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPC056Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 25.2kDa |
| Predicted isoelectric point | 5.7 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | Membrane; Single-pass type I membrane protein. Melanosome |
| Residues | Tyr112~Gly321 (Accession # P50281) with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
基质金属蛋白酶14(MMP14)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YAIQGLKWQ HNEITFCIQN YTPKVGEYAT YEAIRKAFRV WESATPLRFR EVPYAYIREG HEKQADIMIF FAEGFHGDST PFDGEGGFLA HAYFPGPNIG GDTHFDSAEP WTVRNEDLNG NDIFLVAVHE LGHALGLEHS SDPSAIMAPF YQWMDTENFV LPDDDRRGIQ QLYGGESGFP TKMPPQPRTT SRPSVPDKPK NPTYGPNICD G
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YAIQGLKWQ HNEITFCIQN YTPKVGEYAT YEAIRKAFRV WESATPLRFR EVPYAYIREG HEKQADIMIF FAEGFHGDST PFDGEGGFLA HAYFPGPNIG GDTHFDSAEP WTVRNEDLNG NDIFLVAVHE LGHALGLEHS SDPSAIMAPF YQWMDTENFV LPDDDRRGIQ QLYGGESGFP TKMPPQPRTT SRPSVPDKPK NPTYGPNICD G
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
基质金属蛋白酶14(MMP14)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-7973R ATE1精氨酸tRNA的蛋白转移酶1抗体 浓度1mg/ml
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-7975R ATXN7L1共济失调蛋白7样1抗体 浓度1mg/ml
yb-6915R AUTS2孤独症易感基因2蛋白抗体（自闭症相关蛋白1） 浓度1mg/ml
yb-7974R ATX2脊髓小脑共济失调2型蛋白抗体 浓度1mg/ml
yb-9101R ARSH芳香基硫酸酯酶H抗体 浓度1mg/ml
yb-6476R ACF1溴区结构域相邻锌指蛋白1A抗体 浓度1mg/ml
yb-6477R Apo-1载脂蛋白1抗体 浓度1mg/ml
yb-11039R Amisyn突触融合结合蛋白6抗体 浓度1mg/ml
yb-8585R Arginase 1精氨酸酶1抗体 浓度1mg/ml
yb-9532R AKD1腺苷酸激酶结构域蛋白1抗体 浓度1mg/ml
yb-11180R ABCA7三磷酸腺苷结合盒转运蛋白7抗体 浓度1mg/ml
yb-8629R phospho-Na+/K+-ATPase Alpha1(Ser 943)磷酸化钠钾ATP酶α1多肽抗体 浓度1mg/ml
yb-9570R ATP1A1Na+/K+-ATPase α1 钠钾ATP酶α1抗体 浓度1mg/ml
yb-11195R BAF53b肌动蛋白样蛋白6B抗体 浓度1mg/ml
yb-11318R Ataxin 7脊髓小脑共济失调蛋白7抗体 浓度1mg/ml
yb-11329R phospho-c-Abl (Tyr70)磷酸化非受体酪氨酸激酶c-Abl抗体 浓度1mg/ml
yb-11334R APBA1β淀粉样前体蛋白结合蛋白1抗体（X11α） 浓度1mg/ml
yb-4892R Apolipoprotein E载脂蛋白E抗体 浓度1mg/ml
yb-4821R AFP甲胎蛋白AFP抗体 浓度1mg/ml
yb-9847R AE2阴离子交换蛋白2抗体 浓度1mg/ml
yb-9848R Aconitase 1铁调节蛋白1抗体 浓度1mg/ml
yb-9108R ATAD3A三磷酸腺苷酶家族蛋白3A抗体 浓度1mg/ml
yb-1354R APOJ载脂蛋白J抗体 浓度1mg/ml
yb-1570R APOH载脂蛋白H抗体 浓度1mg/ml
yb-7621R ART4二磷酸腺苷核糖基转移酶4抗体 浓度1mg/ml
yb-9082R AKIRIN2AKIRIN2蛋白抗体 浓度1mg/ml
yb-9304R ARL8BADP核糖基化样因子8B抗体 浓度1mg/ml
yb-9110R ATAD2三磷酸腺苷酶家族蛋白2抗体 浓度1mg/ml
yb-9081R AKIRIN1AKIRIN1蛋白抗体 浓度1mg/ml
yb-9325R ASZ1锚定蛋白样蛋白1抗体 浓度1mg/ml
yb-6875R Adenylate kinase 2腺苷酸激酶2抗体 浓度1mg/ml
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文献和实验相关实验
向热力学稳定状态转变，形成具有生物活性的天然结构[11]。然而在去除变性剂的同时，重组蛋白质在体外折叠，分子间存在大量错误折叠和聚合，复性效率往往很低，包涵体蛋白折叠复性的效率实际上取决于正确折叠过程与聚集过程之间的竞争[1]。对于蛋白质的折叠机制，目前有多种不同的假设，但很多学者认为有一个“熔球态”的中间状态，在“熔球态”中，蛋白质的二级结构已经基本形成，其空间结构也初具规模，再做一些局部调整就可形成正确的立体结构，总之，蛋白质的具体步骤可用下式描述[12、13、14]： 伸展态→中间
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基质金属蛋白酶14(MMP14)重组蛋白
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