PRODUCT SOURCE
Recombinant Mouse VEGF164 Protein,HEK293 expressed,ActiveMax (mouse VEGF164) Ala 27 - Arg 190 (Accession # AAB22253.1 ) was produced in human 293 cells (HEK293) at ACROBiosystems.
MOLECULAR CHARACTERIZATION
Rhesus Macaque EGFR / ErbB1 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 95.3 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 130-140 kDa in SDS-PAGE due to glycosylation.
BIOLOGICAL ACTIVITY
Measured in a cell proliferation assay using human umbilical vein endothelial cells ( HUVEC ). The ED50 for this effect is typically 3.5 ng/mL.
PURITY
>92% as determined by SDS-PAGE.
ENDOTOXIN
Less than 1.0 EU per μg of the mouse VEGF164 by the LAL method.