PRODUCT SOURCE
Recombinant Mouse VEGF120 Protein,HEK293 expressed,ActiveMax (Mouse VEGF120) Ala 27 - Arg 146 (Accession # AAB22254.1 ) was produced in human 293 cells (HEK293) at ACROBiosystems.
MOLECULAR CHARACTERIZATION
rh CMV-GB Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 104 kDa. The predicted N-terminus is Val 23. DTT-reduced Protein migrates as 140-150 kDa in SDS-PAGE due to glycosylation.
BIOLOGICAL ACTIVITY
Measured in a cell proliferation assay using human umbilical vein endothelial cells ( HUVEC ). The ED50 for this effect is typically 5 ng/mL.
PURITY
>95% as determined by SDS-PAGE.
ENDOTOXIN
Less than 1.0 EU per μg of the Mouse VEGF120 by the LAL method.