活性:
The bioactivity is measured by its binding ability to human GH in a SPR assay(Biacore T100). Covalently immobilized Human Growth Hormone R, His Tag (Cat# GHR-H5222) can bind human growth hormone protein with affinity constant of 1.28 ±0.16 nM range.
产品背景:
Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
产品描述:
Human Growth Hormone R, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 28.5 kDa. The predicted N-terminus is Ala 27. The reducing (R) protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.