活性:
(1) Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4, Fc Tag (Cat# CT4-H5255) at 10 μg/mL (100 µl/well), can bind Human B7-1, His Tag (Cat# B71-H5228) with a linear range of 0.04-4 μg/mL.(2) Measured by its binding ability in a functional ELISA against immobilized Human B7-2, His Tag (Cat# CD6-H5223, 1 μg/well). The linear range is 0.04-0.3 μg/mL.
产品背景:
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
产品描述:
This protein carries a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40 kDa. The predicted N-terminus is Ala 37. As a result of glycosylation, the reducing (R) protein migrates as 50-55 kDa, and the non-reducing (NR) protein migrates as 100-110 kDa in SDS-PAGE.