- 询价
- USCN
- MAA260Ra21
- 2025年09月09日
- WB, ICC, IHC-P, IHC-F, ELISA
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
WB, ICC, IHC-P, IHC-F, ELISA
- 宿主：
Mouse
- 克隆性：
多克隆
- 免疫原：
Rattus norvegicus (Rat)
- 规格：
200ug
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文献和实验Western Blotting with Monoclonal Ant
). 2.Aspirate excess PBS. 3.Add 1ml boiling 2X concentrated electrophoresis sample buffer (125mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 1.8% beta-mercaptoethanol). 4.Scrape cells from dish, transfer to a microcentrifuge tube, and boil
Western Blotting with Monoclonal Antibodies
plate rapidly with phosphate-buffered saline (PBS). Aspirate excess PBS. Add 1ml boiling 2X concentrated electrophoresis sample buffer (125mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 1.8% beta-mercaptoethanol).
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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