- ¥847.08
- sigma
- 进口
- P5726-1ML
- 2025年07月11日
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- 详细信息
- 技术资料
- 保存条件：
低温
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
产品名称
Application
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.
Biochem/physiol Actions
This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.
Caution
As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.
Preparation Note
This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.
Features and Benefits
This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
性质
Related Categories Bioactive Small Molecule Alphabetical Index, HIS Select Supporting Products and Reagents, HIS-Select, Kinase/Phosphatase Biology, P-PH,
Phosphatase Inhibitor Cocktails, Phosphatase Inhibitors, Products for UpStream Processes, Protease Inhibitors, Tyrosine Phosphatase (TP), Tyrosine Phosphatase Biology, Tyrosine Phosphatase Inhibitors, 分子生物学, 天然蛋白质样品制备, 生物活性小分子, 磷酸化分析, 纯化和检测, 细胞信号转导和神经科学, 细胞生物学, 翻译后修饰, 蛋白质组学, 重组蛋白表达和分析
更多...
Quality Level PREMIUM
form aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
shipped in wet ice
storage temp. 2-8°C
Application
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.
Biochem/physiol Actions
This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.
Caution
As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.
Preparation Note
This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.
Features and Benefits
This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
性质
Related Categories Bioactive Small Molecule Alphabetical Index, HIS Select Supporting Products and Reagents, HIS-Select, Kinase/Phosphatase Biology, P-PH,
Phosphatase Inhibitor Cocktails, Phosphatase Inhibitors, Products for UpStream Processes, Protease Inhibitors, Tyrosine Phosphatase (TP), Tyrosine Phosphatase Biology, Tyrosine Phosphatase Inhibitors, 分子生物学, 天然蛋白质样品制备, 生物活性小分子, 磷酸化分析, 纯化和检测, 细胞信号转导和神经科学, 细胞生物学, 翻译后修饰, 蛋白质组学, 重组蛋白表达和分析
更多...
Quality Level PREMIUM
form aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
shipped in wet ice
storage temp. 2-8°C
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Phosphatase Inhibitor Cocktail 2 磷酸酶抑制剂2 P5726-1ML
¥847.08
