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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
6个月
- 英文名:
Recombinant Desmoglein 1 (DSG1)
- 库存:
10000
- 供应商:
钰博生物
桥粒芯糖蛋白1(DSG1)重组蛋白
适用生物 Homo sapiens (Human,人)
桥粒芯糖蛋白1(DSG1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 58.6kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
yb-3043R phospho-Akt (Thr450)磷酸化蛋白激酶B抗体 浓度1mg/ml
yb-5551R phospho-AMPK alpha-1 (Thr198)磷酸化腺苷单磷酸活化蛋白激酶α1抗体 浓度1mg/ml
yb-2720R phospho-Akt(Thr308)磷酸化蛋白激酶B抗体 浓度1mg/ml
yb-15407R APOBEC3G脱氧胞苷脱氨酶蛋白抗体 浓度1mg/ml
yb-13637R AKAP13蛋白激酶锚定蛋白13抗体 浓度1mg/ml
yb-13638R AP1M2AP-1μ2抗体 浓度1mg/ml
yb-7348R Allergin-1肥大细胞膜抑制性受体Allergin1抗体 浓度1mg/ml
yb-4915R ACCN1脑钠通道蛋白1抗体 浓度1mg/ml
yb-10563R ARP2/3 subunit 1B肌动蛋白相关蛋白2/3复合亚单位B1抗体 浓度1mg/ml
yb-4780R ABCG2三磷酸腺苷结合转运蛋白G超家族成员2抗体 浓度1mg/ml
yb-8616R alpha 1 Fetoprotein甲胎蛋白抗体 浓度1mg/ml
yb-1370R ATM毛细血管扩张性共济失调症突变蛋白抗体 浓度1mg/ml
yb-12441R ABCF2ATP结合盒转运蛋白2抗体 浓度1mg/ml
yb-12442R ABLIM3肌动蛋白结合蛋白LIM蛋白3抗体 浓度1mg/ml
yb-12443R Acidic Calponin酸性钙调蛋白3抗体 浓度1mg/ml
yb-12447R phospho-ADAM17 (Thr735)磷酸化CD156b抗体 浓度1mg/ml
yb-12448R ADH5乙醇脱氢酶5抗体 浓度1mg/ml
yb-12449R ADH6乙醇脱氢酶6抗体 浓度1mg/ml
yb-12451R AGPAT5溶血磷脂酰基转移酶5抗体 浓度1mg/ml
yb-12452R AIPL1遗传性失明相关蛋白AIPL1抗体 浓度1mg/ml
适用生物 Homo sapiens (Human,人)
桥粒芯糖蛋白1(DSG1)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 58.6kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Desmoglein 1 (DSG1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPA729Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 58.6kDa |
| Predicted isoelectric point | 5.4 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | Cell membrane; Single-pass type I membrane protein. Cell junction › desmosome |
| Residues | Glu710~Val961 (Accession # Q02413) with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-E GRPSNDCLLI YDIEGVGSPA GSVGCCSFIG EDLDDSFLDT LGPKFKKLAD ISLGKESYPD LDPSWPPQST EPVCLPQETE PVVSGHPPIS PHFGTTTVIS ESTYPSGPGV LHPKPILDPL GYGNVTVTES YTTSDTLKPS VHVHDNRPAS NVVVTERVVG PISGADLHGM LEMPDLRDGS NVIVTERVIA PSSSLPTSLT IHHPRESSNV VVTERVIQPT SGMIGSLSMH PELANAHNVI VTERVVSGAG V
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-E GRPSNDCLLI YDIEGVGSPA GSVGCCSFIG EDLDDSFLDT LGPKFKKLAD ISLGKESYPD LDPSWPPQST EPVCLPQETE PVVSGHPPIS PHFGTTTVIS ESTYPSGPGV LHPKPILDPL GYGNVTVTES YTTSDTLKPS VHVHDNRPAS NVVVTERVVG PISGADLHGM LEMPDLRDGS NVIVTERVIA PSSSLPTSLT IHHPRESSNV VVTERVIQPT SGMIGSLSMH PELANAHNVI VTERVVSGAG V
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
桥粒芯糖蛋白1(DSG1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
桥粒芯糖蛋白1(DSG1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-12487R APG8A自噬相关蛋白8A抗体 浓度1mg/ml
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
桥粒芯糖蛋白1(DSG1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-3043R phospho-Akt (Thr450)磷酸化蛋白激酶B抗体 浓度1mg/ml
yb-5551R phospho-AMPK alpha-1 (Thr198)磷酸化腺苷单磷酸活化蛋白激酶α1抗体 浓度1mg/ml
yb-2720R phospho-Akt(Thr308)磷酸化蛋白激酶B抗体 浓度1mg/ml
yb-15407R APOBEC3G脱氧胞苷脱氨酶蛋白抗体 浓度1mg/ml
yb-13637R AKAP13蛋白激酶锚定蛋白13抗体 浓度1mg/ml
yb-13638R AP1M2AP-1μ2抗体 浓度1mg/ml
yb-7348R Allergin-1肥大细胞膜抑制性受体Allergin1抗体 浓度1mg/ml
yb-4915R ACCN1脑钠通道蛋白1抗体 浓度1mg/ml
yb-10563R ARP2/3 subunit 1B肌动蛋白相关蛋白2/3复合亚单位B1抗体 浓度1mg/ml
yb-4780R ABCG2三磷酸腺苷结合转运蛋白G超家族成员2抗体 浓度1mg/ml
yb-8616R alpha 1 Fetoprotein甲胎蛋白抗体 浓度1mg/ml
yb-1370R ATM毛细血管扩张性共济失调症突变蛋白抗体 浓度1mg/ml
yb-12441R ABCF2ATP结合盒转运蛋白2抗体 浓度1mg/ml
yb-12442R ABLIM3肌动蛋白结合蛋白LIM蛋白3抗体 浓度1mg/ml
yb-12443R Acidic Calponin酸性钙调蛋白3抗体 浓度1mg/ml
yb-12447R phospho-ADAM17 (Thr735)磷酸化CD156b抗体 浓度1mg/ml
yb-12448R ADH5乙醇脱氢酶5抗体 浓度1mg/ml
yb-12449R ADH6乙醇脱氢酶6抗体 浓度1mg/ml
yb-12451R AGPAT5溶血磷脂酰基转移酶5抗体 浓度1mg/ml
yb-12452R AIPL1遗传性失明相关蛋白AIPL1抗体 浓度1mg/ml
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文献和实验相关实验
溶液(最常用的盐体系为硫酸铵―水溶液),对蛋白质的构象具有一定的稳定作用,所以在分离后,蛋白质可保持高的活性回收率。目前,利用HPHIC分离的蛋白质有糖蛋白、单抗、酶和基因重组蛋白质等。 HIC在近几年的研究重点: 1.新填料的合成像HPIEC一样,HPHIC的非多孔填料、灌注色谱填料和连续柱床填料相继被合成出来,这些新填料的使用大大提高了蛋白质的分离速度和分离效果。 2.蛋白质的保留机制 蛋白质在HPHIC上是如何进行保留的一直是HPHIC的一个主要研究方向。到目前
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桥粒芯糖蛋白1(DSG1)重组蛋白
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