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- 上海钰博
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- YB589Hu01
- 2025年07月13日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Heme Oxygenase 2, Decycling (HO2)
- 库存：
10000
- 供应商：
钰博生物
- 规格：
50ug
血红素氧合酶2(HO2)重组蛋白
适用生物 Homo sapiens (Human，人)
血红素氧合酶2(HO2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 30.8kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
yb-13797R C19orf7519号染色体开放阅读框75抗体 浓度1mg/ml
yb-11441R Cryptochrome 1隐花色素蛋白1抗体 浓度1mg/ml
yb-11447R CRY2隐花色素蛋白2抗体 浓度1mg/ml
yb-11981R CACNA2钙通道L型α2多肽抗体 浓度1mg/ml
yb-11982R CACNB3L型电压依赖型钙通道β3(L-type Ca++ CPβ3)抗体 浓度1mg/ml
yb-11983R CACNB4L型电压依赖型钙通道β4抗体 浓度1mg/ml
yb-11984R CACNA1G + CACNA1H电压依赖性钙通道CACNA1G+CACNA1H抗体 浓度1mg/ml
yb-11986R CABP1钙结合蛋白1抗体 浓度1mg/ml
yb-11987R CAPON神经型一氧化氮合成酶结合蛋白抗体 浓度1mg/ml
yb-11998R CHRM4毒蕈碱型乙酰胆碱受体M4抗体 浓度1mg/ml
yb-0771R CaMK2 alpha钙/钙调素依赖蛋白激酶2α抗体 浓度1mg/ml
yb-11975R CACYBP周期蛋白结合蛋白抗体 浓度1mg/ml
yb-7786R CDCA2细胞分裂周期蛋白2抗体 浓度1mg/ml
yb-7785R CDC45L细胞分裂周期调控蛋白45抗体 浓度1mg/ml
yb-7780R CAPH染色体相关蛋白H抗体 浓度1mg/ml
yb-7788R CEP76中心体蛋白质76抗体 浓度1mg/ml
yb-7787R CEP152中心体蛋白152抗体 浓度1mg/ml
yb-12425R Casein kinase 1 gamma 2酪蛋白激酶1γ2/casein kinase Iγ1抗体 浓度1mg/ml
yb-8018R CLPTM1唇裂和腭裂相关跨膜蛋白1抗体 浓度1mg/ml
yb-8024R CKLFH8趋化素样因子超家族成员8抗体 浓度1mg/ml
yb-8020R CKLFH1趋化素样因子超家族成员1抗体 浓度1mg/ml
yb-8017R CLN6神经细胞蜡样质脂褐质沉积病蛋白CLN6抗体 浓度1mg/ml
yb-12005R CEP104甘氨酸结合蛋白抗体 浓度1mg/ml
适用生物 Homo sapiens (Human，人)
血红素氧合酶2(HO2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 30.8kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Heme Oxygenase 2, Decycling (HO2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPA589Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 30.8kDa |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | Thr71~Leu306 (Accession # P30519) with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
血红素氧合酶2(HO2)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TTALYFTYSA LEEEMERNKD HPAFAPLYFP MELHRKEALT KDMEYFFGEN WEEQVQCPKA AQKYVERIHY IGQNEPELLV AHAYTRYMGD LSGGQVLKKV AQRALKLPST GEGTQFYLFE NVDNAQQFKQ LYRARMNALD LNMKTKERIV EEANKAFEYN MQIFNELDQA GSTLARETLE DGFPVHDGKG DMRKCPFYAA EQDKGALEGS SCPFRTAMAV LRKPSLQFIL AAGVAL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TTALYFTYSA LEEEMERNKD HPAFAPLYFP MELHRKEALT KDMEYFFGEN WEEQVQCPKA AQKYVERIHY IGQNEPELLV AHAYTRYMGD LSGGQVLKKV AQRALKLPST GEGTQFYLFE NVDNAQQFKQ LYRARMNALD LNMKTKERIV EEANKAFEYN MQIFNELDQA GSTLARETLE DGFPVHDGKG DMRKCPFYAA EQDKGALEGS SCPFRTAMAV LRKPSLQFIL AAGVAL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
血红素氧合酶2(HO2)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-4896R CD66a癌胚抗原相关细胞粘附分子1抗体 浓度1mg/ml
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-13797R C19orf7519号染色体开放阅读框75抗体 浓度1mg/ml
yb-11441R Cryptochrome 1隐花色素蛋白1抗体 浓度1mg/ml
yb-11447R CRY2隐花色素蛋白2抗体 浓度1mg/ml
yb-11981R CACNA2钙通道L型α2多肽抗体 浓度1mg/ml
yb-11982R CACNB3L型电压依赖型钙通道β3(L-type Ca++ CPβ3)抗体 浓度1mg/ml
yb-11983R CACNB4L型电压依赖型钙通道β4抗体 浓度1mg/ml
yb-11984R CACNA1G + CACNA1H电压依赖性钙通道CACNA1G+CACNA1H抗体 浓度1mg/ml
yb-11986R CABP1钙结合蛋白1抗体 浓度1mg/ml
yb-11987R CAPON神经型一氧化氮合成酶结合蛋白抗体 浓度1mg/ml
yb-11998R CHRM4毒蕈碱型乙酰胆碱受体M4抗体 浓度1mg/ml
yb-0771R CaMK2 alpha钙/钙调素依赖蛋白激酶2α抗体 浓度1mg/ml
yb-11975R CACYBP周期蛋白结合蛋白抗体 浓度1mg/ml
yb-7786R CDCA2细胞分裂周期蛋白2抗体 浓度1mg/ml
yb-7785R CDC45L细胞分裂周期调控蛋白45抗体 浓度1mg/ml
yb-7780R CAPH染色体相关蛋白H抗体 浓度1mg/ml
yb-7788R CEP76中心体蛋白质76抗体 浓度1mg/ml
yb-7787R CEP152中心体蛋白152抗体 浓度1mg/ml
yb-12425R Casein kinase 1 gamma 2酪蛋白激酶1γ2/casein kinase Iγ1抗体 浓度1mg/ml
yb-8018R CLPTM1唇裂和腭裂相关跨膜蛋白1抗体 浓度1mg/ml
yb-8024R CKLFH8趋化素样因子超家族成员8抗体 浓度1mg/ml
yb-8020R CKLFH1趋化素样因子超家族成员1抗体 浓度1mg/ml
yb-8017R CLN6神经细胞蜡样质脂褐质沉积病蛋白CLN6抗体 浓度1mg/ml
yb-12005R CEP104甘氨酸结合蛋白抗体 浓度1mg/ml
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文献和实验相关实验
生成素(EPO)转铁蛋白、血红素加氧酶-1等的基因，它们都有一个共同的顺式作用元件(CGTG)，有利于在5’或3’侧翼区的低氧诱导低氧诱导作用因子-1(HIF-1)和低氧反应增强子(HRE)结合，激活靶基因的转录，在低氧浓度下可使重组蛋白大量表达。据报道红细胞生成素启动子在1%氧浓度比在21%氧浓度下活陛增强100倍。这又是一种新的提高表达量的作用机制。 4．提高蛋白表达产量的措施 哺乳动物细胞对培养环境十分敏感，营养和生长因子缺乏、缺氧、病毒感染、毒性代谢
。这些启动子在诱导前后活性可相差4个数量级。另外，在哺乳动物细胞中已发现存在大量在低氧环境中可诱导转录的基因，如编码红细胞生成素(EPO)转铁蛋 白、血红素加氧酶-1等的基因，它们都有一个共同的顺式作用元件(CGTG )，有利于在5’或3’侧翼区的低氧诱导低氧诱导作用因子-1(HIF-1)和低氧反应增强子(HRE)结合，激活靶基因的转录，在低氧浓度下可使重组蛋白大量表达。据报道红细胞生成素启动子在 1%氧浓度比在21%氧浓度下活陛增强100倍。这又是一种新的提高表达量的作用机制。 2、宿主
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血红素氧合酶2(HO2)重组蛋白
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