DNA提取试剂盒(DNA Extractor® SP Kit) 本试剂盒可从血清或血浆中高效提取DNA片段。 因基于碘化钠(NaI)法,整个实验操作可在单一的微型离心管中完成,且并不使用具有危险性的酚/氯仿。近期,不同癌症患者(如肺癌、乳腺癌和结肠癌患者)血清或血浆中的肿瘤特异性基因的扩增检测已经实现(参考文献见下)。本试剂盒由于其高品质和高产率使其成为一个功能强大的目标DNA检测分析的预处理试剂 [特点] 1. 可从小量血清和血浆中提取高产率DNA(约100%) 2. 操作安全:不需要酚/氯仿 3. 最低限度的污染:整个提取的DNA可以在一个单一的离心管中完成。 4. 彻底清除血液中的血脂 5. 提取过程中样本变异率低 [试剂盒组成] 1. 酶反应液 1 vial x 10 mL 2. 蛋白消化液 1 vial x 250 uL 3. 碘化钠溶液 1 vial x 15 mL 4. 乙醇溶液 1 vial x 30 mL 5. 清洗液A 1 vial x 50 mL 6. 清洗液B 1 vial x 50 mL [应用数据] Result of amplification of p53-Exon 5 region extracted from human serum and plasma (此地插入图片) [标准操作步骤] l 100ul sample of serum or plasma: +200ul of enzyme reaction solution and mix briefly +5ul of protein digestion solution and vortex. Incubate at 56℃ for 10min. +300ul of sodium lodide solution and mix briefly +600ul of alcohol solution and vortex.Incubate at room temperature for 10min Centrifuge at 12000~20000 *g for 10 min,at room temperature. l Pellet: +1ml of washing solution (A) to the pellet and vortex. Centrifuge at 12000~20000 *g for 5 min,at room temperature. l Pellet: +1ml of washing solution (B) to the pellet and vortex. Centrifuge at 12000~20000 *g for 5 min,at room temperature. l Pellet: Dry thye pellet well for about 5 min,at about 65℃ Adequate volume (10~20ul) of TE(pH8.0) or D.W and vortex. Dissolve the pellet completely with cortex mixer and incubate at about 65℃ for 3~5min. l DNA solution [参考文献] 1) Ishizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994). 2) Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M.A. and Pastorino, U.: Clin. Cancer Res., 5, 2689 (1999). 3) Silva, J. M., Dominguez, G., Garcia, J.M., Gonzalesz, R., Villanueva, M.J., avarro, F., Proencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999). 4) Shao, Z.M., Wu, J., Shen, Z.Z. and Nguyen, M.: Clin. Cancer Res., 7, 2222 (2001). 产品信息品名 样本 用途 规格 DNA Extractor SP® Kit 血清、血浆 遗传学研究 50tests DNA Extractor® Kit 血浆、生物制药 遗传学研究 50tests DNA Extractor® WB Kit 全血、细胞培养液、组织 全血DNA提取 50tests DNA Extractor® WB-Rapid Kit 全血、细胞培养液、组织 遗传学研究 20tests 200tests DNA Isolator PS Kit 石蜡切片,标本 遗传学研究 100tests DNA Isolator PS-Rapid Reagent 石蜡切片,标本 100tests DNA Extractor® FM Kit 毛发、血迹 法医学研究 50tests p53 Primer Exon 5 人 100tests p53 Primer Exon 7 人 100tests mt DNA Extractor® WB Kit 人全血 mtDNA提取 25tests mt DNA Extractor® CT Kit 细胞培养液、组织 mtDNA提取 25tests 还有更多,欢迎联络!
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