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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20C
- 英文名:
Valerian Root HP-TLC Kit w/ Standards
- 供应商:
上海汇谱仪器有限公司
- 规格:
kit
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文献和实验7.5. 3. 10% (w/v) NaN3 . 4. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.3. Fractionation of Wnt3A CM 2.3.1 Step 1: Blue Sepharose 1. Sample: 1–4 L Wnt3A CM as prepared in Section 2.2 . 2. Column: Blue Sepharose HP
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
and 0.25% (w/v) Bromophenol Blue) 100 bp Size Standards DNA ladder (1 mg/ml) 50 µl 1 M Tris-HCl (pH 8.0) 5 µl 0.5 M EDTA (pH 8.0) 5 µl Loading Buffer 440 µl 500 µl Method 1. Cast a 2% agarose gel (1X TAE) with four combs (50
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