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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20C
- 英文名:
GOLDENSEAL STANDARDS KIT(P) (AHP Verified)
- 供应商:
上海汇谱仪器有限公司
- 规格:
2 x 25mg
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文献和实验siRNAs to a number of important human genes. These siRNAs have actually been tested and verified to reduce target mRNA levels >70%.General Design GuidelinesIf you prefer to design your own siRNAs, you can choose siRNA target sites in a variety
Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
restriction enzyme digests and 1kb ladder and lambda standards. If large amounts are needed follow the procedure for a large scale plasmid preparations when the plasmid/insert is verified. Transformation of ligation mixes: Day 1 1.Verify selection
Cloning of Taq polymerase-amplified PCR products
out the gel slice containing the PCR product and melt it at 65°C in 2 volumes of 6 M NaI. 4) Add 1.5 volumes Binding Buffer (provided in the S.N.A.P. ™ MiniPrep Kit). 5) Load solution (no more than 1 ml at a time) from Step 3 onto a S
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