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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
RESVERATROL ANALOG 2002(LG)
- 供应商:
上海汇谱仪器有限公司
- 规格:
1mg
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文献和实验; biotinylated c?di?GMP conjugate; 6?(2?cyanoethylthio)guanosine; c?di?GMP mono?6?thioated analog GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library
同位素法测定底物磷酸化活性方法 Phosphorylation of Substrates
in the hydrolysis of the analog ATP within 1 min (Chaudhary et al. 2002). Therefore, we have chosen to apply this technique to cell lysates or fractions. Here we describe two approaches to detect direct substrates of a protein kinase. The first approach involves
实验试剂 1. Recombinant kinase (II) 2. [γ-32 P]ATP analog (I II) 3. Sepharose CL-6B beads (I) 4. Lipofectamine (I) 5. Agonist (I), e.g., epidermal
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