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- ASB-00020075-005
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20C
- 英文名：
TAXANE (TAXOL) STD MIXTURE(13 compound mix)(SH)
- 供应商：
上海汇谱仪器有限公司
- 规格：
0.5mL
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文献和实验Tubulin Polymerization with GTP/GMPCPP/Taxol
rotor at 90K for 5' at 2¡C. Incubate supernatant at 37¡C for 1'-2'. Now there are two options : I . Add taxol stepwise to equimolar as follows (for 1 mg/ml tubulin): (Pipet in the taxol and immediately flick the tube to mix
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
fluorescent polarity marked taxol-stabilized microtubules to determine polarity, as described later. 1. Coat flow cell with a mixture of 0.25 mg/ml casein and kinesin (5-50 µg/ml) for 3'.. 2. Rinse out unbound material with 80 µl BRB80 + 1 mM DTT
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
with a mixture of 0.25 mg/ml casein and kinesin (5-50 µg/ml) for 3'. 2. Rinse out unbound material with 80 µl BRB80 + 1 mM DTT + 1 mM MgATP + 10 µM taxol + 1X OS Mix. 3. Flow in 30-50 µl of 10 ug/ml polarity marked taxol microtubules diluted from a 1
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